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The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum

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Abstract

Our study aims to assess the implication of WRKY transcription factor in the molecular mechanisms of grapevine adaptation to salt and water stresses. In this respect, a full-length VvWRKY2 cDNA, isolated from a Vitis vinifera grape berry cDNA library, was constitutively over-expressed in Nicotiana tabacum seedlings. Our results showed that transgenic tobacco plants exhibited higher seed germination rates and better growth, under both salt and osmotic stress treatments, when compared to wild type plants. Furthermore, our analyses demonstrated that, under stress conditions, transgenic plants accumulated more osmolytes, such as soluble sugars and free proline, while no changes were observed regarding electrolyte leakage, H2O2, and malondialdehyde contents. The improvement of osmotic adjustment may be an important mechanism underlying the role of VvWRKY2 in promoting tolerance and adaptation to abiotic stresses. Principal component analysis of our results highlighted a clear partition of plant response to stress. On the other hand, we observed a significant adaptation behaviour response for transgenic lines under stress. Taken together, all our findings suggest that over-expression of VvWRKY2 gene has a compelling role in abiotic stress tolerance and, therefore, would provide a useful strategy to promote abiotic stress tolerance in grape via molecular-assisted breeding and/or new biotechnology tools.

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Abbreviations

CI:

Confidence interval

FW:

Fresh weight

H2O2 :

Hydrogen peroxide

PCA:

Principal component analysis

ROS:

Reactive oxygen species

TBA:

Thiobarbituric acid

TF:

Transcription factor

WT:

Wild type

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Acknowledgements

We gratefully thank Mrs Sana Louati, Mrs Leila Hjaiej and Mr. Mounir Triki for proofreading the present paper.

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Authors and Affiliations

  1. Laboratory of Extremophile Plants, Centre of Biotechnology of Borj-Cedria, P. O. Box 901, 2050, Hammam-Lif, Tunisia

    Rim Mzid, Walid Zorrig, Karim Ben Hamed, Yosra Damak & Mohsen Hanana

  2. Molecular and Cellular Screening Processes, Centre of Biotechnology of Sfax, PB ‘1177’, 3018, Sfax, Tunisia

    Rayda Ben Ayed & Mariem Ayadi

  3. Institut des Sciences de la Vigne et du Vin (ISVV), UMR 1287 “Ecophysiologie et Génomique Fonctionnelle de la Vigne”, 210 Chemin de Leysotte, 33882, Villenave d’Ornon, France

    Virginie Lauvergeat

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  1. Rim Mzid
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Correspondence to Rim Mzid.

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Mzid, R., Zorrig, W., Ben Ayed, R. et al. The grapevine VvWRKY2 gene enhances salt and osmotic stress tolerance in transgenic Nicotiana tabacum. 3 Biotech 8, 277 (2018). https://doi.org/10.1007/s13205-018-1301-4

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