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Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica

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Abstract

The effect of abiotic (salicylic acid, jasmonic acid and methyl jasmonate) and biotic (chitosan and yeast extract) elicitors on the growth and xanthone accumulation in two hairy root clones of Gentiana dinarica Beck. was studied. The obtained results showed that clone 3 was more responsive to elicitor treatment than clone D. The production of dominant xanthone norswertianin-1-O-primeveroside was not significantly affected by either of the abiotic elicitor tested but was stimulated with chitosan treatment. The highest concentrations of all elicitors strongly increased the content of xanthone aglycone norswertianin, but simultaneously reducing the production of its glycoside norswertianin-1-O-primeveroside. The most efficient in enhancing norswertianin production was a 7-day treatment with salicylic acid (200 μM) and chitosan (50 mg l− 1), which yielded a 7.7- and a 24-fold increase in norswertianin content, respectively. In addition, treatment with biotic elicitors caused the occurrence of new xanthone compounds that were not detected in other samples. Free radical scavenging activity of xanthones was carried out by DPPH assay, and norswertianin showed the strongest activity.

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Abbreviations

ASA:

Ascorbic acid

CH:

Chitosan

DPPH:

1,1-Diphenyl-2-picrylhydrazyl

DW:

Dry weight

JA:

Jasmonic acid

MeJA:

Methyl jasmonate

NOR:

Norswertianin

NOR-1-O-PRIM:

Norswertianin-1-O-primeveroside

SA:

Salicylic acid

YE:

Yeast extract

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Acknowledgements

The present work was supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia, Grant No. 173015.

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Authors and Affiliations

  1. Institute for Biological Research “Siniša Stanković”, University of Belgrade, Bulevar despota Stefana 142, Belgrade, 11060, Serbia

    Dijana Krstić-Milošević, Branka Uzelac, Dragan Vinterhalter & Branka Vinterhalter

  2. Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000, Belgrade, Serbia

    Teodora Janković

Authors
  1. Dijana Krstić-Milošević
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  2. Teodora Janković
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  3. Branka Uzelac
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Contributions

BV produced and maintained in vitro cultures and designed and supervised the whole study. DKM and TJ provided plant material, performed phytochemical analysis and wrote the manuscript. DV helped with experimental design and performed statistical analysis of the data. BU contributed to the writing and correction of the manuscript.

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Correspondence to Dijana Krstić-Milošević.

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Communicated by: Silvia Moreno.

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Krstić-Milošević, D., Janković, T., Uzelac, B. et al. Effect of elicitors on xanthone accumulation and biomass production in hairy root cultures of Gentiana dinarica . Plant Cell Tiss Organ Cult 130, 631–640 (2017). https://doi.org/10.1007/s11240-017-1252-1

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  • DOI: https://doi.org/10.1007/s11240-017-1252-1

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