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Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation

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Abstract

The effect of abiotic and biotic elicitors (methyl jasmonate, chitosan, salicylic acid, Agrobacterium, and yeast extract) at various concentrations on total isoflavonoid accumulation was studied in the hairy root cultures of Pueraria candollei. All elicitors stimulated isoflavonoid production. Yeast extract (0.5 mg/ml) was the most efficient giving total isoflavonoids at 60 ± 1 mg/g dry wt, which was 4.5-fold higher than control hairy roots on day 3 of elicitation.

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Acknowledgments

This work was accomplished through a grant by the Office of the Higher Education Commission. Latiporn Udomsuk (CHE 510636) was supported by the CHE-Ph.D. scholarship. The Thailand Research Fund (RSA5280012) is greatly acknowledged.

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Authors and Affiliations

  1. Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand

    Latiporn Udomsuk, Kanokwan Jarukamjorn & Waraporn Putalun

  2. Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, 812-8582, Japan

    Hiroyuki Tanaka

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  1. Latiporn Udomsuk
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  2. Kanokwan Jarukamjorn
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  3. Hiroyuki Tanaka
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  4. Waraporn Putalun
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Correspondence to Waraporn Putalun.

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Udomsuk, L., Jarukamjorn, K., Tanaka, H. et al. Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation. Biotechnol Lett 33, 369–374 (2011). https://doi.org/10.1007/s10529-010-0417-3

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  • DOI: https://doi.org/10.1007/s10529-010-0417-3

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