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Multiple shoot induction and jasmonic versus salicylic acid driven elicitation for enhanced andrographolide production in Andrographis paniculata

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Abstract

Multiple shoot induction at an average of (15.25 ± 2.128 and 16.16 ± 3.851 shoots/explant) was obtained from Andrographis paniculata cotyledonary node explants on Murashige and Skoog’s (MS) medium supplemented with 2.0 mg/L 6-Benzylaminopurine (BAP) and Zeatin, respectively after 8 weeks of culture. Further proliferation of cultures in MS + BAP medium at a maximum of 150 ± 18.40 multiple shoots was obtained from single cotyledonary node explants after three subculture (84 days) in vitro passage. Elicitation as yield enhancement strategy, a protocol was developed using multiple shoots to treat the cultures with jasmonic acid (JA) and salicylic acid (SA). Various concentrations of JA (1, 5, 10, 25, 50, 75 and 100 µM) and SA (10, 20, 50 and 100 µM) were used for elicitation. Treatment of JA at 1.0 µM concentration resulted in 1.322 % dry weight (DW) with 2.6-fold increase in andrographolide production after fifth week compared to 0.508 % DW in untreated control. However, JA treatment at 25 and 50 µM promoted 3.3, 3.0-fold enhancement in andrographolide production (1.624 and 1.481 % DW), respectively; after eighth week compared to control. Treatment of 10, 20 and 50 µM SA resulted in 3.0, 3.4 and 3.1-fold andrographolide content (1.479, 1.654 and 1.483 % DW), increase after eighth week, respectively; compared to control (0.478 % DW). This is the first report on elicitation of A. paniculata multiple shoot cultures using signal molecules (JA and SA). The present findings may be helpful for in vitro manipulation and enhanced production of andrographolides.

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Abbreviations

BAP:

6-Benzylaminopurine

DW:

Dry weight

FW:

Fresh weight

HPLC:

High performance liquid chromatography

JA:

Jasmonic acid

Kn:

Kinetin

MS:

Murashige and Skoog (1962)

SA:

Salicylic acid

Zt:

Zeatin

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Acknowledgments

Authors would like to thank sponsors University Grants Commission (UGC), New Delhi OU-DST-PURSE project Department of Science and Technology (DST), New Delhi for financial support. Mr. Mohd. Zaheer thanks UGC and DST (OU-DST-PURSE), New Delhi for the Research Fellowships.

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  1. Centre for Plant Molecular Biology (CPMB), Osmania University, Hyderabad, 500007, India

    Mohd Zaheer & Charu Chandra Giri

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Correspondence to Charu Chandra Giri.

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Zaheer, M., Giri, C.C. Multiple shoot induction and jasmonic versus salicylic acid driven elicitation for enhanced andrographolide production in Andrographis paniculata . Plant Cell Tiss Organ Cult 122, 553–563 (2015). https://doi.org/10.1007/s11240-015-0787-2

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