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High variability in the tissue culture response of root-tips of Allium ascalonicum individuals and optimization of the regeneration procedure

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Abstract

This study assessed the shoot regeneration capacity of root-tips isolated from single seed-derived individual plants, obtained from a wild, open-pollinated micropopulation of shallot (Allium ascalonicum). Considerable variation was observed in the regeneration capacities of individual lines, ranging from 0.93 to 100 %, and a mean bud number per root explant between 0.09 and 20.67. One line was found to be superior, and was chosen for protocol optimization, focusing on the 2,4-D/BA ratio, duration of the CI phase and light conditions. The application of the optimized protocol to other lines, selected for their variable regeneration capacities, enhanced the process of regeneration and shortened the time required to obtain healthy plantlets, even in inferior lines. However, highly responsive lines remained superior, indicating that differences at the individual level must not be overlooked. The conditions employed in this study may serve as a base to facilitate the application of molecular breeding methods in shallot.

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Abbreviations

ANOVA:

Analysis of variance

BA:

6-Benzylaminopurine

BFC:

Bud-forming capacity

CI:

Callus induction

CIM:

Callus induction medium

2,4-D:

2,4-Dichlorophenoxyacetic acid

IBA:

Indole-3-butyric acid

Kin:

6-Furfurylaminopurine

MS:

Murashige and Skoog mineral solution

PGR:

Plant growth regulator

RI:

Regeneration induction

RIM:

Regeneration induction medium

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Acknowledgments

The authors would like to express their gratitude to the Ministry of Education, Science and Technological Development of the Republic of Serbia for financial support through Contract Nos. 173015 and 173030.

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Authors and Affiliations

  1. Department of Plant Physiology, Institute for Biological Research “Siniša Stanković”, University of Belgrade, Despot Stefan Boulevard 142, 11 060, Belgrade, Serbia

    Ljiljana Tubić, Jelena Milojević, Nabil Ghalawenji, Nevena Mitić & Snežana Zdravković-Korać

  2. Department of Biology and Ecology, Faculty of Sciences, University of Novi Sad, Trg Dositeja Obradovića 3, 21 000, Novi Sad, Serbia

    Goran Anačkov & Ružica Igić

Authors
  1. Ljiljana Tubić
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  2. Goran Anačkov
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  3. Jelena Milojević
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  4. Nabil Ghalawenji
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  5. Nevena Mitić
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  6. Ružica Igić
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  7. Snežana Zdravković-Korać
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Correspondence to Ljiljana Tubić.

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Tubić, L., Anačkov, G., Milojević, J. et al. High variability in the tissue culture response of root-tips of Allium ascalonicum individuals and optimization of the regeneration procedure. Plant Cell Tiss Organ Cult 118, 101–110 (2014). https://doi.org/10.1007/s11240-014-0465-9

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  • DOI: https://doi.org/10.1007/s11240-014-0465-9

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