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Overexpression of Os2H16 enhances resistance to phytopathogens and tolerance to drought stress in rice

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Abstract

Os2H16, a rice gene of unknown function, has been previously reported to be upregulated in response to infection by Xanthomonas oryzae pv. oryzae. In this study, expression patterns of Os2H16 were analyzed, demonstrating that expression of Os2H16 was dramatically induced by both bacterial and fungal infection as well as by drought stress, but repressed by salt treatment. To further investigate the role of Os2H16 in plant defense responses to abiotic and biotic stresses, transgenic lines of rice were developed. In comparison with wild-type rice, transgenic lines overexpressing Os2H16 show enhanced tolerance to bacterial blight and sheath blight disease, respectively caused by Xanthomonas oryzae pv. oryzae and Rhizoctonia solani. On the contrary, Os2H16 knockdown lines were more susceptible to both pathogens. Consistent with their individual phenotypes, upon inoculation, the expression of defense-related marker genes were elevated in Os2H16 overexpression individuals than in wild-type, while they were significantly reduced in Os2H16 knockdown lines. We also show that Os2H16 overexpression lines display enhanced tolerance to drought stress and elevated induction of drought-related genes, compared to wild-type rice. Os2H16 knockdown lines were more sensitive to drought stress and exhibited reduced induction of drought-related genes. Our study provides the first functional characterization of the rice Os2H16 gene, and suggests that Os2H16 positively modulate plant defense to abiotic and biotic stress.

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Acknowledgments

This study was supported by the National Program of Transgenic Variety Development of China (2011ZX08009-004, 2011ZX08001-002), National Natural Science Foundation of China (Grant No. 31071381) and the Taishan Scholar Programme of Shandong Province. We thank L. Xiong for providing Osbzip23 mutant line, G. Chen for providing RS105 strains as well as J. Yu for providing R. solani strain. We also thank F. Yao for management of rice in the field.

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  1. State Key Laboratory of Crop Biology, Shandong Provincial Key Laboratory of Agricultural Microbiology, Shandong Agricultural University, Tai an, 271018, Shandong, People’s Republic of China

    Ning Li, Lingguang Kong, Wenhui Zhou, Xia Zhang, Shutong Wei, Xinhua Ding & Zhaohui Chu

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  1. Ning Li
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  2. Lingguang Kong
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  3. Wenhui Zhou
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  4. Xia Zhang
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  5. Shutong Wei
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  6. Xinhua Ding
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  7. Zhaohui Chu
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Correspondence to Xinhua Ding or Zhaohui Chu.

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Ning Li and Lingguang Kong contributed equally to this work.

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Li, N., Kong, L., Zhou, W. et al. Overexpression of Os2H16 enhances resistance to phytopathogens and tolerance to drought stress in rice. Plant Cell Tiss Organ Cult 115, 429–441 (2013). https://doi.org/10.1007/s11240-013-0374-3

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  • DOI: https://doi.org/10.1007/s11240-013-0374-3

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