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Identification of major quantitative trait loci qSBR11-1 for sheath blight resistance in rice

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Abstract

Sheath blight caused by Rhizoctonia solani Kühn is one of the important diseases of rice, resulting in heavy yield loss in rice every year. No rice line resistant to sheath blight has been identified till date. However, in some rice lines a high degree of resistance to R. solani has been observed. An indica rice line, Tetep, is a well documented source of durable and broad spectrum resistance to rice blast as well as quantitative resistance to sheath blight. The present study identified genetic loci for quantitative resistance to sheath blight in rice line Tetep. A mapping population consisting of 127 recombinant inbred lines derived from a cross between rice cultivars HP2216 (susceptible) and Tetep (resistant to sheath blight) was evaluated for sheath blight resistance and other agronomic traits for 4 years across three locations. Based on sheath blight phenotypes and genetic map with 126 evenly distributed molecular markers, a quantitative trait loci (QTLs) contributing to sheath blight resistance was identified on long arm of chromosome 11. Two QTL mapping approaches i.e., single marker analysis and composite interval mapping in multi environments were used to identify QTLs for sheath blight resistance and agronomical traits. The QTL qSBR11-1 for sheath blight resistance was identified between the marker interval RM1233 (26.45 Mb) to sbq33 (28.35 Mb) on chromosome 11. This region was further narrowed down to marker interval K39516 to sbq33 (~0.85 Mb) and a total of 154 genes were predicted including 11 tandem repeats of chitinase genes which may be responsible for sheath blight resistance in rice line Tetep. A set of 96 varieties and a F2 population were used for validation of markers linked to the QTL region. The results indicate that there is very high genetic variation among varieties at this locus, which can serve as a starting point for allele mining of sheath blight resistance.

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Acknowledgments

T. R. Sharma is thankful to the Indian Council of Agricultural Research, New Delhi, India for the financial assistance.

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Authors and Affiliations

  1. National Research Centre on Plant Biotechnology, Indian Agricultural Research Institute, New Delhi, 110012, India

    V. Channamallikarjuna, H. Sonah, H. C. Upreti, N. K. Singh & T. R. Sharma

  2. Directorate of Rice Research, Hyderabad, Andhra Pradesh, India

    M. Prasad

  3. Central Rice Research Institute, Cuttack, Orissa, India

    G. J. N. Rao

  4. Devi Ahilya University, Khandwa Road, Indore, 452017, India

    S. Chand

  5. Banasthali University, Jaipur, Rajasthan, 304022, India

    H. Sonah

Authors
  1. V. Channamallikarjuna
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  2. H. Sonah
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  3. M. Prasad
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  4. G. J. N. Rao
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  5. S. Chand
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  6. H. C. Upreti
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  7. N. K. Singh
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  8. T. R. Sharma
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Corresponding author

Correspondence to T. R. Sharma.

Additional information

V. Channamallikarjuna and H. Sonah have contributed equally.

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Channamallikarjuna, V., Sonah, H., Prasad, M. et al. Identification of major quantitative trait loci qSBR11-1 for sheath blight resistance in rice. Mol Breeding 25, 155–166 (2010). https://doi.org/10.1007/s11032-009-9316-5

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  • DOI: https://doi.org/10.1007/s11032-009-9316-5

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