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Phenotypic gain from introgression of two QTL, qSB9-2 and qSB12-1, for rice sheath blight resistance

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Abstract

F2:3 families from crosses between three rice indica introgression lines and their common japonica recurrent parent were used to evaluate two quantitative trait loci (QTL) for sheath blight (SB) resistance. Three selected TeQing-into-Lemont backcross introgression lines (TILs) were more resistant than their susceptible parent (Lemont) in inoculated field plots, and were molecularly verified to contain TeQing alleles at qSB9-2 and/or qSB12-1. F2 individuals homozygous for qSB9-2 and qSB12-1 provided F2:3 families that fit four genotypic classes: containing the resistant TeQing allele for qSB9-2 TQ alone, qSB12-1 TQ alone, both qSB9-2 TQ and qSB12-1 TQ , and neither SB QTL introgression. By comparing the SB resistance of these four genotypic classes in micro-chamber evaluations and inoculated field plots, the phenotypic values of the QTL were measured. Under both study conditions, disease resistance ranked qSB9-2 + qSB12-1 > qSB9-2 > qSB12-1 > no QTL, with both qSB9-2 and qSB12-1 acting as dominant resistance genes. In micro-chamber studies, qSB9-2 TQ reduced disease an average of 1.0 disease index units and qSB12-1 TQ by 0.7 using a scale of 0–9. Field effects of qSB9-2 TQ and qB12-1 TQ were less pronounced, with average phenotypic gains of 0.5 and 0.2 units, respectively. TIL:642 proved to contain qSB9-2 TQ in an introgression so small it was tagged by just RM205 on the tip of chromosome 9. These studies verify that the indica introgression of qSB9-2 TQ or qSB12-1 TQ can measurably improve resistance to sheath blight disease in a highly susceptible tropical japonica cultivar, and fine-mapped the qSB9-2 locus. Markers presently verified as linked to these QTL can support marker-assisted breeding to improve disease resistance.

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Abbreviations

CSSL:

Chromosome segment substitution line

LSM:

Least squared mean

MAS:

Marker-assisted selection

Mbp:

Mega base pair

QTL:

Quantitative trait locus/loci

RIL:

Recombinant inbred line

SB:

Sheath blight

SBR:

Sheath blight resistance

SSR:

Simple sequence repeat

TIL:

TeQing-into-Lemont introgression line (equivalent to chromosome segment substitution line)

References

  • Adam GC (1988) Thanatephorus cucumeris (Rhizoctonia solani) a species of wide host range. In: Sidhu GS (ed) Advances in plant pathology, vol 6. Genetics of plant pathogenic fungi. Academic Press, New York, NY, pp 535–552

    Google Scholar 

  • Belmar SB, Jones RK, Starr JL (1987) Influence of crop rotation on inoculum density of Rhizoctonia solani and sheath blight incidence in rice. Phytopathology 77:1138–1143

    Article  Google Scholar 

  • Chen ZX, Zhou JH, Xu JY, Tong YH, Tang SZ, Wang ZB, Jing RM, Ling B, Tang J, Pan XB (2000) A preliminary study on resources of resistance to rice sheath blight. Chin J Rice Sci 14:15–18

    Google Scholar 

  • Damicone JP, Patel MV, Moore WF (1993) Density of sclerotia of Rhizoctonia solani and incidence of sheath blight in rice fields in Mississippi. Plant Dis 77:257–260

    Article  Google Scholar 

  • Eizenga GC, Lee FN, Rutger JN (2002) Screening Oryza species plants for rice sheath blight resistance. Plant Dis 86:808–812

    Article  Google Scholar 

  • Fjellstrom RG, McClung AM, Shank AR (2006) SSR markers closely linked to the Pi-z locus are useful for selection of blast resistance in a broad array of rice germplasm. Mol Breed 17:149–157

    Article  CAS  Google Scholar 

  • Fu D, Chen L, Yu G, Liu Y, Lou Q, Mei H, Ziong L, Li M, Xu X, Luo L (2011) QTL mapping of sheath blight resistance in a deep-water rice cultivar. Euphytica 180:209–218. doi:10.1007/s10681-011-0366-5

    Article  Google Scholar 

  • Han YP, Xing YZ, Chen ZX, Gu SL, Pan ZB, Chen XL, Zhang QF (2002) Mapping QTLs for horizontal resistance to sheath blight in an elite rice restorer line, Minghui 63. Acta Genet Sinica 29:565–570

    Google Scholar 

  • Jia Y, Correa-Victoria FJ, McClung AM, Zhu L, Liu G, Wamishe Y, Xie J, Marchetti MA, Pinson SRM, Rutger JN, Correll JC (2007) Rapid determination of rice cultivar responses to the sheath blight pathogen Rhizoctonia solani using micro-chamber screening method. Plant Dis 91:485–489

    Article  Google Scholar 

  • Kunihiro Y, Qian Q, Sato H, Teng S, Zeng DL, Fujimoto K, Zhu LH (2002) QTL analysis of sheath blight resistance in rice (Oryza sativa L.). Acta Genet Sin 29:50–55

    PubMed  CAS  Google Scholar 

  • Li ZK, Pinson SRM, Marchetti MA (1995) Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani). Theor Appl Genet 91:382–388

    CAS  Google Scholar 

  • Liu YF, Chen ZY, Ji JA, Liu YZ (2006) Analysis of resistance to sheath blight on the commercial cultivars and new potential breeding lines of Jiangsu Province. Jiangsu Agric Sci 1:27–28

    Google Scholar 

  • Liu G, Jia Y, Correa-Victoria FJ, Prado GA, Yeater KM, McClung AM, Correll JC (2009) Mapping quantitative trait loci responsible for resistance to sheath blight in rice. Phytopathology 99:1078–1084

    Article  PubMed  CAS  Google Scholar 

  • Loan LC, Du PV, Li Z (2004) Molecular dissection of quantitative resistance of sheath blight in rice (Oryza sativa L.). Omonrice 12:1–12

    Google Scholar 

  • Marchetti MA, Bollich CN (1991) Quantification of the relationship between sheath blight severity and yield loss in rice. Plant Dis 75:773–775

    Article  Google Scholar 

  • Mew TW, Collyn B, Pamplona P (2004) Applying rice seed-associated antagonistic bacteria to manage rice sheath blight in developing countries. Plant Dis 88:557–564

    Article  Google Scholar 

  • Ou SH (1985) Rice diseases, 2nd edn. Commonwealth Mycological Inst, Kew

    Google Scholar 

  • Pan XB, Rush MC (1997) Studies in the U.S. on genetics and breeding of resistance to rice sheath blight. J Jiangsu Agric Coll 18:57–63

    Google Scholar 

  • Park DS, Sayler RJ, Hong YG, Nam MH, Yang YN (2008) A method for inoculation and evaluation of rice sheath blight disease. Plant Dis 92:25–29

    Article  Google Scholar 

  • Pinson SRM, Capdevielle FM, Oard JH (2005) Confirming QTLs and finding additional loci conditioning sheath blight resistance in rice (Oryza sativa L.) using recombinant inbred lines. Crop Sci 45:503–510

    Article  CAS  Google Scholar 

  • Pinson SRM, Oard JH, Groth D, Miller R, Marchetti MA, Shank AR, Jia MH, Jia Y, Fjellstrom RG, Li Z (2008) Registration of TIL:455, TIL:514, and TIL:642, three rice germplasm lines containing introgressed sheath blight resistance alleles. J Plant Reg 2:251–254

    Article  Google Scholar 

  • Pinson SRM, Liu G, Jia MH, Jia Y, Fjellstrom RG, Sharma A, Wang Y, Tabien RE, Li Z (2011) Registration of a rice gene mapping population consisting of ‘TeQing’-into-‘Lemont’ (TIL) backcross introgression lines. J Plant Reg (submitted)

  • Rush MC, Lee FN (1992) Sheath blight. In: Webster RK, Gunnell PS (eds) Compendium of rice diseases. APS Press, St. Paul, pp 22–23

    Google Scholar 

  • SAS Institute (2004) The SAS system for windows, V 9.1.3. SAS Inst, Cary

    Google Scholar 

  • Sato H, Ideta O, Audo I, Kunihiro Y, Hirabayashi H, Iwano M, Miyasaka A, Nemoto H, Imbe T (2004) Mapping QTLs for sheath blight resistance in the rice line WSS2. Breed Sci 54:265–271

    Article  CAS  Google Scholar 

  • Savary S, Castilla NP, Elazegui FA, McLaren CG, Ynalvez MA, Teng PS (1995) Direct and indirect effects of nitrogen supply and disease source structure on rice sheath blight spread. Phytopathology 85:959–965

    Article  Google Scholar 

  • Sharma A, McClung AM, Pinson SRM, Kepiro JL, Shank AR, Tabien RE, Fjellstrom RG (2009) Genetic mapping of sheath blight resistance QTLs within tropical japonica rice cultivars. Crop Sci 49:256–264

    Article  CAS  Google Scholar 

  • Srinivasachary, Willocquet L, Savary S (2011) Resistance to rice sheath blight (Rhizoctonia solani Kühn) [(teleomorph: Thanatephorus cucumeris (A.B. Frank) Donk.] disease: current status and perspectives. Euphytica 178:1–22

    Article  Google Scholar 

  • Tang JB, Ma BT, Wang LZ, Li P, Zheng AP, Chen H (2002) Biological control of rice sheath blight with Trichoderma. Chin J Rice Sci 16:63–66

    Google Scholar 

  • Wamishe YA, Jia Y, Singh P, Cartwright RD (2007) Identification of field isolates of Rhizoctonia solani to detect quantitative resistance in rice under greenhouse conditions. Agric Chin 1:361–367

    Article  Google Scholar 

  • Xie XW, Xu MR, Zang JP, Sun Y, Zhu LH, Xu JL, Zhou YL, Li ZK (2008) Genetic background and environmental effects on expression of QTL for sheath blight resistance in reciprocal introgression lines of rice. Acta Agron Sin 34:1885–1893

    Article  CAS  Google Scholar 

  • Zou JH, Pan XB, Chen ZX, Xu JY, Lu JF, Zhai WX, Zhu LH (2000) Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars (Oryza sativa L.). Theor Appl Genet 101:569–575

    Article  CAS  Google Scholar 

  • Zuo SM, Yin YJ, Zhang L, Zhang YF, Chen ZX, Pan XB (2007) Breeding value and further mapping of a QTL qSB-11 conferring the rice sheath blight resistance. Chin J Rice Sci 21:136–142

    CAS  Google Scholar 

  • Zuo S, Zhang L, Wang H, Yin Y, Zhang Y, Chen Z, Ma Y, Pan X (2008) Prospect of the QTL-qSB-9Tq utilized in molecular breeding program of japonica rice against sheath blight. J Genet Genomics 35:499–505

    Article  PubMed  CAS  Google Scholar 

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Acknowledgments

This research was supported in part by the USDA Cooperative State Research, Education and Extension Service—National Research Initiative—Applied Plant Genomics Program entitled “RiceCAP: A coordinated research, education, and extension project for the application of genomic discoveries to improve rice in the United States” (USDA/CSREES grant 2004 35317 14867). The authors thank Ms. Piper Roberts, pathology technician at the USDA-ARS Rice Research Unit, Beaumont, TX for field plot management and disease ratings.

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Authors and Affiliations

  1. AgriLife Research and Extension Center, Texas A&M University, 1509 Aggie Drive, Beaumont, TX, 77713-8530, USA

    Y. Wang & R. E. Tabien

  2. Everglades Research and Education Center, 3200 E. Palm Beach Road., Belle Glade, FL, 33430-4702, USA

    Y. Wang

  3. Rice Research Unit, USDA-ARS, 1509 Aggie Drive, Beaumont, TX, 77713-8530, USA

    S. R. M. Pinson & R. G. Fjellstrom

  4. Dale Bumpers National Rice Research Center, USDA-ARS, 2890 Highway 130 East, Stuttgart, AR, 72160-5647, USA

    R. G. Fjellstrom

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  1. Y. Wang
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  2. S. R. M. Pinson
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  3. R. G. Fjellstrom
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  4. R. E. Tabien
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Correspondence to S. R. M. Pinson.

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Wang, Y., Pinson, S.R.M., Fjellstrom, R.G. et al. Phenotypic gain from introgression of two QTL, qSB9-2 and qSB12-1, for rice sheath blight resistance. Mol Breeding 30, 293–303 (2012). https://doi.org/10.1007/s11032-011-9619-1

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