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Overexpression of ALDH2B8, an aldehyde dehydrogenase gene from grapevine, sustains Arabidopsis growth upon salt stress and protects plants against oxidative stress

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Abstract

Aldehyde dehydrogenases (ALDHs) belong to a family of NAD (P)+-dependent enzymes that catalyze the oxidation of various toxic aldehydes to carboxylic acids. They have been reported to play important roles in plant responses to various stresses. Here we report on the isolation of a grapevine ALDH gene, which is rapidly induced in response to NaCl treatment. When transiently expressed in Arabidopsis protoplasts, grapevine ALDH2B8 was found to be localized in mitochondria. Transgenic Arabidopsis plants overexpressing grapevine ALDH2B8 showed sustained growth upon salt stress and increased tolerance against oxidative stress, which was correlated with decreased accumulation of reactive oxygen specie and malondialdehyde derived from cellular lipid peroxidation. In addition, the transgenic line had longer roots and higher chlorophyll content than the wild type under high salinity conditions. Taken together, we suggest that grapevine ALDH2B8 is involved in plant responses to oxidative and salt stress.

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Abbreviations

ABA:

Abscisic acid

ALDHs:

Aldehyde dehydrogenases

cDNA:

Complementary deoxyribonucleic acid

DAB:

Diaminobenzidine

FW:

Fresh weight

GFP:

Green fluorescence protein

H2O2 :

Hydrogen peroxide

MDA:

Malondialdehyde

MS:

Murashige and Skoog medium

NAD (P):

Nicotinamide adenine dinucleotide (phosphate)

NBT:

Nitro blue tetrazolium

O2 − :

Superoxide anion

qRT-PCR:

Quantitative reverse transcriptase-polymerase chain reaction

ROS:

Reactive oxygen specie

SOD:

Superoxide dismutase

WT:

Wild type

T-NOS:

Nopaline synthase terminator

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Acknowledgments

The authors thank Dr. Zhangjun Fei for critical revision of this manuscript. This work was supported by the National Natural Science Foundation of China (31071782), 948 Project from Ministry of Agriculture of China (2012-S12), and Chinese Universities Scientific Fund (QN2011056).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology in Arid Areas, College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xiaozhao Xu, Rongrong Guo, Chenxia Cheng, Hongjing Zhang, Yucheng Zhang & Xiping Wang

  2. Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China, Ministry of Agriculture, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Xiaozhao Xu, Rongrong Guo, Chenxia Cheng, Hongjing Zhang, Yucheng Zhang & Xiping Wang

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  1. Xiaozhao Xu
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  2. Rongrong Guo
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  3. Chenxia Cheng
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  4. Hongjing Zhang
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  5. Yucheng Zhang
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Correspondence to Xiping Wang.

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Xu, X., Guo, R., Cheng, C. et al. Overexpression of ALDH2B8, an aldehyde dehydrogenase gene from grapevine, sustains Arabidopsis growth upon salt stress and protects plants against oxidative stress. Plant Cell Tiss Organ Cult 114, 187–196 (2013). https://doi.org/10.1007/s11240-013-0314-2

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