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VpWRKY3, a biotic and abiotic stress-related transcription factor from the Chinese wild Vitis pseudoreticulata

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Abstract

Chinese wild grapevine Vitis pseudoreticulata accession ‘Baihe-35-1’ is identified as the precious resource with multiple resistances to pathogens. A directional cDNA library was constructed from the young leaves inoculated with Erysiphe necator. A total of 3,500 clones were sequenced, yielding 1,727 unigenes. Among them, 762 unigenes were annotated and classified into three classes, respectively, using Gene Ontology, including 22 ESTs related to transcription regulator activity. A novel WRKY transcription factor was isolated from the library, and designated as VpWRKY3 (GenBank Accession No. JF500755). The full-length cDNA is 1,280 bp, encoding a WRKY protein of 320 amino acids. VpWRKY3 is localized to nucleus and functions as a transcriptional activator. QRT-PCR analysis showed that the VpWRKY3 specifically accumulated in response to pathogen, salicylic acid, ethylene and drought stress. Overexpression of VpWRKY3 in tobacco increased the resistance to Ralstonia solanacearum, indicating that VpWRKY3 participates in defense response. Furthermore, VpWRKY3 is also involved in abscisic acid signal pathway and salt stress. This experiment provided an important basis for understanding the defense mechanisms mediated by WRKY genes in China wild grapevine. Generation of the EST collection from the cDNA library provided valuable information for the grapevine breeding.

Key message We constructed a cDNA library from Chinese wild grapevine leaves inoculated with powdery mildew. VpWRKY3 was isolated and demonstrated that it was involved in biotic and abiotic stress responses.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 30971972).

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Authors and Affiliations

  1. College of Horticulture, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Ziguo Zhu, Jiangli Shi, Jiangling Cao, Mingyang He & Yuejin Wang

  2. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (Northwest Region), Ministry of Agriculture, Beijing, People’s Republic of China

    Ziguo Zhu, Jiangli Shi, Jiangling Cao, Mingyang He & Yuejin Wang

  3. State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    Ziguo Zhu, Jiangli Shi, Jiangling Cao, Mingyang He & Yuejin Wang

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  1. Ziguo Zhu
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  2. Jiangli Shi
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  3. Jiangling Cao
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  4. Mingyang He
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Correspondence to Yuejin Wang.

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Communicated by A. Feher.

Z. Zhu and J. Shi contributed equally to this work.

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Zhu, Z., Shi, J., Cao, J. et al. VpWRKY3, a biotic and abiotic stress-related transcription factor from the Chinese wild Vitis pseudoreticulata . Plant Cell Rep 31, 2109–2120 (2012). https://doi.org/10.1007/s00299-012-1321-1

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  • DOI: https://doi.org/10.1007/s00299-012-1321-1

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