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Efficiency of direct and indirect shoot organogenesis in different genotypes of Rosa hybrida

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Abstract

To optimize indirect regeneration (IR) and direct regeneration (DR) in Rosa hybrida cv. Apollo different explant types and different concentrations of plant growth regulators were investigated. Among the different auxins studied and over all explant types, 10 µM 2,4-dichlorophenoxyacetic acid (2,4-D) promoted the highest frequency of callus production for IR. The highest frequency of regeneration (60.8 %) was obtained when calli were transferred to Murashige and Skoog medium supplemented with 2.5 µM thidiazuron (TDZ) and 2 µM gibberellic acid. The highest frequency of regeneration (80.2 %) for DR was obtained from leaves cultured on the medium containing 10 µM TDZ. The efficiency of IR and DR were compared in four different rose cultivars including ‘Apollo’, ‘Black Baccara’, ‘Maroussia’ and ‘Amanda’. The frequency of regeneration in all four cultivars was significantly higher in DR compared to IR. Also shoots regenerated by DR appeared earlier than the shoots regenerated by IR. The results of flow cytometry showed that the shoots derived from IR to DR were tetraploid like the original cultivars.

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Abbreviations

MS medium:

Murashige and Skoog medium

2,4,5-T:

2,4,5-Trichlorophenoxyacetic acid

2,4-D:

2,4-Dichlorophenoxyacetic acid

TDZ:

Thidiazuron

BA:

Benzyladenine

GA3 :

Gibberellic acid 3

NAA:

α-Naphthalene acetic acid

IR:

Indirect regeneration

DR:

Direct regeneration

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Acknowledgments

This work was part of a PhD thesis on “Study of gene transformation and regeneration in roses” in Science and Research Branch, Islamic Azad University, funded by Agricultural Biotechnology Research Institute of Iran (ABRII) (Project number: 12-05-05-01-8704-88003). Authors wish to thank Prof A. V. Roberts from University of East London, UK, for his constructive comments on the manuscript.

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Authors and Affiliations

  1. Department of Tissue Culture and Gene Transformation, Agricultural Biotechnology Research Institute of Iran (ABRII), P.O. Box 31535-1897, Mahdasht Road, Karaj, Iran

    Leila Pourhosseini, Maryam Jafarkhani Kermani & Ali Akbar Habashi

  2. Department of Horticultural Science, Science and Research Branch, Islamic Azad University (IAU), Tehran, Iran

    Leila Pourhosseini, Maryam Jafarkhani Kermani & Ahmad Khalighi

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  1. Leila Pourhosseini
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  2. Maryam Jafarkhani Kermani
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  3. Ali Akbar Habashi
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  4. Ahmad Khalighi
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Correspondence to Maryam Jafarkhani Kermani.

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Pourhosseini, L., Kermani, M.J., Habashi, A.A. et al. Efficiency of direct and indirect shoot organogenesis in different genotypes of Rosa hybrida . Plant Cell Tiss Organ Cult 112, 101–108 (2013). https://doi.org/10.1007/s11240-012-0210-1

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