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Effects of Agrobacterium rhizogenes strains and other parameters on production of isoflavonoids in hairy roots of Pueraria candollei Grah. ex Benth. var. candollei

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Abstract

Using several explants of Pueraria candollei Grah. ex Benth. var. candollei and two strains of Agrobacterium rhizogenes (ATCC 15834 and 43057), hairy root cultures were established. Including 100 μM acetosyringone in the culture medium enhanced frequency of hairy root induction by up to 58 %. Subsequently, effects of inoculum size (IS) and temperature on growth and production of isoflavonoids in hairy roots were determined. Conditions of 1 % IS and 32 °C promoted the highest accumulation of total isoflavonoid content, up to 31.0 ± 22.6 mg/g dry weight (DW), in hairy roots. Moreover, culture of hairy roots at 32 °C decreased browning of hairy roots. Furthermore, this temperature promoted accumulation of the secondary metabolite daidzein; whereas, hairy root cultures at the stationary phase accumulated higher amounts of the isoflavonoid puerarin rather than daidzein.

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Abbreviations

AS:

Acetosyringone

B5:

Gamborg’s B5 medium (Gamborg et al. 1968)

DW:

Dry weight

FW:

Fresh weight

IS:

Inoculum size

MS:

Murashige and Skoog medium (Murashige and Skoog 1962)

References

  • Boonsnongcheep P, Korsangruang S, Soonthornchareonnon N, Chintapakorn Y, Saralamp P, Prathanturarug S (2010) Growth and isoflavonoid accumulation of Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tissue Organ Cult 101:119–126

    Article  CAS  Google Scholar 

  • Chandra S, Chandra R (2011) Engineering secondary metabolite production in hairy roots. Phytochem Rev 10:371–395

    Article  CAS  Google Scholar 

  • Chansakaow S, Ishikawa T, Sekine K, Okeda M, Higuchi Y, Chaichantipyuth C (2000a) Identification of deoxymiroestrol as the actual rejuvenating principle of “kwao keur”, Pueraria mirifica. The known miroestrol may be an artifact. J Nat Prod 63:173–175

    Article  PubMed  CAS  Google Scholar 

  • Chansakaow S, Ishikawa T, Sekine K, Okeda M, Higuchi Y, Kudo M, Chaichantipyuth C (2000b) Isoflavonoids from Pueraria mirifica and their estrogenic activity. Planta Med 66:572–575

    Article  PubMed  CAS  Google Scholar 

  • Cherdshewasart W, Sriwatcharakul S (2007) Major isoflavonoid contents of the 1-year-cultivated phytoestrogen-rich herb, Pueraria mirifica. Biosci Biotechnol Biochem 71:2527–2533

    Article  PubMed  CAS  Google Scholar 

  • Cherdshewasart W, Sutjit W (2008) Correlation of antioxidant activity and major isoflavonoid contents of the phytoestrogen-rich Pueraria mirifica and Pueraria lobata tubers. Phytomedicine 15:38–43

    Article  PubMed  CAS  Google Scholar 

  • Dehghan E, Häkkinen S, Oksman-Caldentey K-M, Shahriari Ahmadi F (2012) Production of tropane alkaloids in diploid and tetraploid plants and in vitro hairy root cultures of Egyptian henbane (Hyoscyamus muticus L.). Plant Cell Tissue Organ Cult 110:35–44

    Google Scholar 

  • Furner IJ, Huffman GA, Amasino RM, Garfinkel DJ, Gordon MP, Nester EW (1986) An Agrobacterium transformation in the evolution of the genus Nicotiana. Nature 319:422–427

    Article  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158

    Article  PubMed  CAS  Google Scholar 

  • Geng L, Niu L, Gresshoff P, Shu C, Song F, Huang D, Zhang J (2012) Efficient production of Agrobacterium rhizogenes-transformed roots and composite plants in peanut (Arachis hypogaea L.). Plant Cell Tissue Organ Cult 109:491–500

    Article  CAS  Google Scholar 

  • Georgiev M, Pavlov A, Bley T (2007) Hairy root type plant in vitro systems as sources of bioactive substances. Appl Microbiol Biotechnol 74:1175–1185

    Article  PubMed  CAS  Google Scholar 

  • He X, Blount J, Ge S, Tang Y, Dixon R (2011) A genomic approach to isoflavone biosynthesis in kudzu (Pueraria lobata). Planta 233:843–855

    Article  PubMed  CAS  Google Scholar 

  • Ingham JL, Tahara S, Dziedzic SZ (1986) A chemical investigation of Pueraria mirifica roots. Z Naturforsch C 41:403–408

    CAS  Google Scholar 

  • Jeong GT, Park DH, Ryu HW, Lee WT, Park K, Kang CH, Hwang B, Woo JC (2002) Optimum conditions for transformed Panax ginseng hairy roots in flask culture. Appl Biochem Biotech 98–100:1129–1139

    Article  Google Scholar 

  • Jeong GT, Park DH, Ryu HW, Hwang B, Woo JC (2004) Effects of inoculum conditions on growth of hairy roots of Panax ginseng C.A. Meyer. Appl Biochem Biotechnol 116:1193–1203

    Article  Google Scholar 

  • Keung WM (2002) Pueraria: the genus Pueraria. Taylor & Francis, New York

    Google Scholar 

  • Kintzios S, Makri O, Pistola E, Matakiadis T, Ping Shi H, Economou A (2004) Scale-up production of puerarin from hairy roots of Pueraria phaseoloides in an airlift bioreactor. Biotechnol Lett 26:1057–1059

    Article  PubMed  CAS  Google Scholar 

  • Korsangruang S, Soonthornchareonnon N, Chintapakorn Y, Saralamp P, Prathanturarug S (2010) Effects of abiotic and biotic elicitors on growth and isoflavonoid accumulation in Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tissue Organ Cult 103:333–342

    Article  CAS  Google Scholar 

  • Liu C, Zhu J, Liu Z, Li L, Pan R, Jin L (2002) Exogenous auxin effects on growth and phenotype of normal and hairy roots of Pueraria lobata (Willd.) Ohwi. Plant Growth Regul 38:37–43

    Article  CAS  Google Scholar 

  • Mathur A, Gangwar A, Mathur A, Verma P, Uniyal G, Lal R (2010) Growth kinetics and ginsenosides production in transformed hairy roots of American ginseng-Panax quinquefolium L. Biotechnol Lett 32:457–461

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Ozyigit II, Kahraman MV, Ercan O (2007) Relation between explant age, total phenols and regeneration response in tissue cultured cotton (Gossypium hirsutum L.). Afr J Biotechnol 6:3–8

    CAS  Google Scholar 

  • Pietrosiuk A, Syktowska-Baranek K, Wiedenfeld H, Wolinowska R, Furmanowa M, Jaroszyk E (2006) The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm. Plant Cell Rep 25:1052–1058

    Article  PubMed  CAS  Google Scholar 

  • Saleh NM, Thuc LV (2009) Assessment of hairy roots induction in Solenostemon scutellarioides leaves by different strains of Agrobacterium rhizogenes. Afr J Biotechnol 8:3519–3523

    Google Scholar 

  • Sevόn N, Oksman-Caldentey KM (2002) Agrobacterium rhizogenes-mediated transformation: root cultures as a source of alkaloids. Planta Med 68:859–868

    Article  Google Scholar 

  • Sheikholeslam SN, Weeks DP (1987) Acetosyringone promotes high efficiency transformation of Arabidopsis thaliana explants by Agrobacterium tumefaciens. Plant Mol Biol 8:291–298

    Article  CAS  Google Scholar 

  • Shi HP, Kintzios S (2003) Genetic transformation of Pueraria phaseoloides with Agrobacterium rhizogenes and puerarin production in hairy roots. Plant Cell Rep 21:1103–1107

    Article  PubMed  CAS  Google Scholar 

  • Shi HP, Long YY, Sun TS, Tsang E (2011) Induction of hairy roots and plant regeneration from the medicinal plant Pogostemon cablin. Plant Cell Tissue Organ Cult 107:251–260

    Article  CAS  Google Scholar 

  • Sidwa-Gorycka M, Krolicka A, Orlita A, Malinski E, Golebiowski M, Kumirska J, Chromik A, Biskup E, Stepnowski P, Lojkowska E (2009) Genetic transformation of Ruta graveolens L. by Agrobacterium rhizogenes: hairy root cultures a promising approach for production of coumarins and furanocoumarins. Plant Cell Tissue Organ Cult 97:59–69

    Article  CAS  Google Scholar 

  • Stachel SE, Messens E, Van Montagu M, Zambryski P (1985) Identification of the signal molecules produced by wounded plant cells that activate T-DNA transfer in Agrobacterium tumefaciens. Nature 318:624–629

    Article  Google Scholar 

  • Sucontphunt A, De-Eknamkul W, Nimmannit U, Dan Dimitrijevich S, Gracy RW (2011) Protection of HT22 neuronal cells against glutamate toxicity mediated by the antioxidant activity of Pueraria candollei var. mirifica extracts. J Nat Prod 65:1–8

    CAS  Google Scholar 

  • Sykłowska-Baranek K, Pietrosiuk A, Gawron A, Kawiak A, Łojkowska E, Jeziorek M, Chinou I (2012) Enhanced production of antitumour naphthoquinones in transgenic hairy root lines of Lithospermum canescens. Plant Cell Tissue Organ Cult 108:213–219

    Article  Google Scholar 

  • Tao J, Li L (2006) Genetic transformation of Torenia fournieri L. mediated by Agrobacterium rhizogenes. S. Afr J Bot 72:211–216

    Article  Google Scholar 

  • Thanonkeo S, Panichajakul S (2006) Production of isoflavones, daidzein and genistein in callus cultures of Pueraria candollei Wall. ex Benth. var. mirifica. Songklanakarin. J Sci Technol 28:45–53

    Google Scholar 

  • Thimmaraju R, Venkatachalam L, Bhagyalakshmi N (2008) Morphometric and biochemical characterization of red beet (Beta vulgaris L.) hairy roots obtained after single and double transformations. Plant Cell Rep 27:1039–1052

    Article  PubMed  CAS  Google Scholar 

  • Trisomboon H, Malaivijitnond S, Watanabe G, Cherdshewasart W, Taya K (2006) The estrogenic effect of Pueraria mirifica on gonadotrophin levels in aged monkeys. Endocrine 29:129–134

    Article  PubMed  CAS  Google Scholar 

  • Udomsuk L, Jarukamjorn K, Tanaka H, Putalun W (2009) Isoflavonoid production in a hairy roots culture of Pueraria candollei. Z Naturforsch C 64:687–691

    PubMed  CAS  Google Scholar 

  • Udomsuk L, Jarukamjorn K, Tanaka H, Putalun W (2011) Improved isoflavonoid production in Pueraria candollei hairy root cultures using elicitation. Biotechnol Lett 33:369–374

    Article  PubMed  CAS  Google Scholar 

  • Urasopon N, Hamada Y, Asaoka K, Cherdshewasart W, Malaivijitnond S (2007) Pueraria mirifica, a phytoestrogen-rich herb, prevents bone loss in orchidectomized rats. Maturitas 56:322–331

    Article  PubMed  CAS  Google Scholar 

  • Wang YM, Wang JB, Luo D, Jia JF (2001) Regeneration of plants from callus cultures of roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi. Cell Res 11:279–284

    Article  PubMed  CAS  Google Scholar 

  • Yusakul G, Putalun W, Udomsin O, Juengwatanatraku T, Chaichantipyuth C (2011) Comparative analysis of the chemical constituents of two varieties of Pueraria candollei. Fitoterapia 82:203–207

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by the Office of the High Education Commission and Mahidol University under the National Research Universities Initiative and Thailand Research Fund (TRF-DBG4980009).

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Authors and Affiliations

  1. Department of Pharmaceutical Botany, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand

    Pimsiri Danphitsanuparn, Panitch Boonsnongcheep, Thanaphol Boriboonkaset & Sompop Prathanturarug

  2. Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, Thailand

    Yupyn Chintapakorn

Authors
  1. Pimsiri Danphitsanuparn
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  2. Panitch Boonsnongcheep
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  3. Thanaphol Boriboonkaset
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  4. Yupyn Chintapakorn
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  5. Sompop Prathanturarug
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Correspondence to Sompop Prathanturarug.

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Danphitsanuparn, P., Boonsnongcheep, P., Boriboonkaset, T. et al. Effects of Agrobacterium rhizogenes strains and other parameters on production of isoflavonoids in hairy roots of Pueraria candollei Grah. ex Benth. var. candollei . Plant Cell Tiss Organ Cult 111, 315–322 (2012). https://doi.org/10.1007/s11240-012-0196-8

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  • DOI: https://doi.org/10.1007/s11240-012-0196-8

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