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The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm.

  • Genetic Transformation and Hybridization
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Abstract

Hairy root cultures of Lithospermum canescens were established using three strains of Agrobacterium rhizogenes: ATCC 15834, LBA 9402 and NCIB 8196. Eight lines resulting from infection with A. rhizogenes ATCC 15834 demonstrated sufficient biomass increase and were submitted to further investigations. The contents of acetylshikonin (ACS) and isobutyrylshikonin (IBS) in transformed hairy roots made up ca. 10% of those observed in natural roots of L. canescens (24.35 and 14.48 mg g−1 DW, respectively). One line, Lc1-D, produced the largest amounts of ACS (2.72 mg g−1 DW) and IBS (0.307 mg g−1 DW). Traces of pyrrolizidine alkaloids (PA), canescine and canescenine, were found in all lines of transformed hairy roots.

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Abbreviations

ACS:

acetylshikonin

DW:

dry weight

IBS:

isobutyrylshikonin

PA:

pyrrolizidine alkaloids

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Acknowledgements

This investigation was supported by a research grant PBZ-KBN-092/P05/2003 from the State Committee of Scientific Research. We are thankful to Dr. Branka Barl, chief scientist, New Era Nutrition, Inc., Edmonton, Alberta, for plants and seeds of L. canescens. We are also grateful to Professor Mondher Jaziri from the Laboratory of Biotechnology and Plant Morphology, Free University of Brussels for the bacterial strain A. rhizogenes NCIB 8196. We thank Mr Ireneusz Rudnicki, Photodepartment of Medical University of Warsaw, Poland for photographic documentation.

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Authors and Affiliations

  1. Department of Biology and Pharmaceutical Botany, Medical University of Warsaw, ul. Banacha 1, 02-097, Warsaw, Poland

    A. Pietrosiuk, K. Sykłowska-Baranek, M. Furmanowa & E. Jaroszyk

  2. Pharmazeutisches Institut der Universität, An der Immenburg 4, D-53121, Bonn, Germany

    H. Wiedenfeld

  3. Department of Microbiology, Medical University of Warsaw, ul. Oczki 3, 02-007, Warsaw, Poland

    R. Wolinowska

Authors
  1. A. Pietrosiuk
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  2. K. Sykłowska-Baranek
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  3. H. Wiedenfeld
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  4. R. Wolinowska
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  5. M. Furmanowa
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  6. E. Jaroszyk
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Correspondence to A. Pietrosiuk.

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Communicated by M. E. Horn

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Pietrosiuk, A., Sykłowska-Baranek, K., Wiedenfeld, H. et al. The shikonin derivatives and pyrrolizidine alkaloids in hairy root cultures of Lithospermum canescens (Michx.) Lehm.. Plant Cell Rep 25, 1052–1058 (2006). https://doi.org/10.1007/s00299-006-0161-2

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  • DOI: https://doi.org/10.1007/s00299-006-0161-2

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