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Overexpression of a cytosolic glyceraldehyde-3-phosphate dehydrogenase gene OsGAPC3 confers salt tolerance in rice

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Abstract

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a highly conserved glycolytic enzyme that plays an important role in carbon economy. However, recent analyses of GAPDH demonstrate that GAPDH is a multifunctional protein that has roles in various cellular functions. In this study, three putative cytosolic GAPDH protein sequences (OsGAPC1–3) were identified from the rice (Oryza sativa) genome. The OsGAPC family has similar exon–intron structures. OsGAPCs transcripts were highly present in seedling shoots and roots, booting leaves, and flowers, but are at low levels in booting culms. Three OsGAPC genes are responsive to all the abiotic stresses including osmotic (20% PEG 6000), salt (200 mM NaCl), heat (42°C), abscisic acid (50 μM) and methyl viologen (50 μM) treatments. Transient expression of GFP-OsGAPC3 fusion protein in onion epidermal cells revealed that OsGAPC3 was indeed a cytosolic protein. One of the representative OsGAPC genes, OsGAPC3, which was induced most significantly by salt stress, was over-expressed in japonica rice Zhonghua 11 under the control of a ubiquitin promoter. Transgenic rice plants overexpressing OsGAPC3 showed enhanced tolerance to salt stress. Furthermore, we found that OsGAPC3 could alleviate the salt toxicity through the regulation of hydrogen peroxide (H2O2) levels. Taken together, these results indicate that OsGAPC3 plays important roles in salt stress tolerance in rice.

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Abbreviations

ABA:

Abscisic acid

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

H2O2 :

Hydrogen peroxide

MS:

Murashige and Skoog medium

MV:

Methyl viologen

PEG:

Polyethylene glycol

RT–PCR:

Reverse transcription-polymerase chain reaction

ROS:

Reactive oxygen species

WT:

Wild-type

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Acknowledgments

This work was supported by National Natural Science Foundation of China (Grant # 30821064), Major State Basic Research Program (2007CB108701) and National Transgenic Research Project (2009zx08009-018B) to YT Lu.

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice and Key Lab of MOE for Plant Developmental Biology, College of Life Sciences, Wuhan University, 430072, Wuhan, China

    Xiu-Hong Zhang, Xiao-Lan Rao, Hai-Tao Shi, Rong-Jun Li & Ying-Tang Lu

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  1. Xiu-Hong Zhang
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  2. Xiao-Lan Rao
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  3. Hai-Tao Shi
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Correspondence to Ying-Tang Lu.

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Zhang, XH., Rao, XL., Shi, HT. et al. Overexpression of a cytosolic glyceraldehyde-3-phosphate dehydrogenase gene OsGAPC3 confers salt tolerance in rice. Plant Cell Tiss Organ Cult 107, 1–11 (2011). https://doi.org/10.1007/s11240-011-9950-6

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