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Induction of somatic embryogenesis from female flower buds of elite Schisandra chinensis

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Abstract

Somatic embryogenesis (SE) was induced in female flower buds from mature Schisandra chinensis cultivar ‘Hongzhenzhu’. Somatic embryo structures were induced at a low frequency from unopened female flower buds and excised unopened on Murashige and Skoog (MS) agar medium containing 4.0 mg l−1 2,4-dichlorophenoxyacetic acid (2,4-D). Friable embryogenic calli were induced from somatic embryo structures after three to four subcultures on initiation medium. The frequencies of mature somatic embryo germination and plantlet conversion were low, but increased in the presence of gibberellic acid (GA3). Some germinated somatic embryos could form friable embryogenic calli on medium without plant growth regulators (PGRs). The germination and conversion frequencies of somatic embryos from embryogenic calli induced using PGR-free medium were higher than for somatic embryos from embryogenic calli induced on medium containing 2,4-D. Most somatic embryos from 2,4-D-induced embryogenic calli had trumpet-shaped embryos, and most somatic embryos from PGR-free medium–induced embryogenic calli had two or three cotyledons. Histological observation indicated that two- and three-cotyledon embryos had defined shoot primordia, but most of the trumpet-shaped embryos yielded plantlets that lacked or had poorly developed meristem tissue. Cytological and random amplification of polymorphic DNA (RAPD) analyses indicated no evidence of genetic variation in the plantlets of somatic embryo origin.

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Acknowledgments

The authors would like to acknowledge the Fundamental Research Funds for the Central Universities (DL09CA12), National Natural Science Foundation of China (No. 30671701), and Sponsoring the excellent doctor degree dissertation in Northeast Forestry University (OPTP10-NEFU).

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Authors and Affiliations

  1. Key Laboratory of Forest Improvement and Biotechnology, Ministry of Education, Northeast Forestry University, 150040, Harbin, People’s Republic of China

    Jing Li Yang, Chuan Ping Yang, Gui Feng Liu & Cheng Hao Li

  2. Research Center for Molecular Developmental Biology, Key Lab of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, CAS, 100093, Beijing, People’s Republic of China

    Yu Da Niu

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  1. Jing Li Yang
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  2. Yu Da Niu
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  3. Chuan Ping Yang
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  4. Gui Feng Liu
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  5. Cheng Hao Li
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Correspondence to Cheng Hao Li.

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Yang, J.L., Niu, Y.D., Yang, C.P. et al. Induction of somatic embryogenesis from female flower buds of elite Schisandra chinensis . Plant Cell Tiss Organ Cult 106, 391–399 (2011). https://doi.org/10.1007/s11240-011-9935-5

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  • DOI: https://doi.org/10.1007/s11240-011-9935-5

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