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Somatic embryogenesis from zygotic embryos of Schisandra chinensis

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  • Published:
Biologia Plantarum

Abstract

We describe the multi-step regeneration system of medicinal plant Schisandra chinensis (Turcz.) Baill. The seeds were pre-treated with 0.005 μM thidiazuron. Subsequently the zygotic embryos of the early heart stage were cultured on medium with 50 μM of 2,4-dichlorophenoxyacetic acid (2,4-D) and after three weeks the embryogenic calli were transferred to a medium with 10 μM of 2,4-D and 4 μM of 6-benzyladenine and were sub-cultured at the 4-week intervals. Abscisic acid (30 μM) and polyethyleneglycol (3 %) significantly influenced the synchronization of development of the somatic embryos (SEs) to the globular stage. The following culture on a medium without growth regulators resulted in full developed cotyledonary stage SEs. Indole-3-butyric acid (0.05 μ) contributed to their rapid conversion to plantlets.

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Abbreviations

ABA:

abscisic acid

AC:

activated charcoal

BA:

6-benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

indole-3-butyric acid

MS medium:

Murashige and Skoog medium

PEG:

polyethyleneglycol 4000

SEs:

somatic embryos

TDZ:

thidiazuron

WV5:

Westvaco medium

ZEs:

zygotic embryos

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Authors and Affiliations

  1. Department of Botany and Plant Physiology, Mendel University of Agriculture and Forestry, Zemedelska 1, CZ-61300, Brno, Czech Republic

    A. Smiskova, H. Vlasinova & L. Havel

Authors
  1. A. Smiskova
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  2. H. Vlasinova
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  3. L. Havel
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Corresponding author

Correspondence to L. Havel.

Additional information

This experimental work was supported by project No. 521/02/1129 of the Grant Agency of the Czech Republic.

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Smiskova, A., Vlasinova, H. & Havel, L. Somatic embryogenesis from zygotic embryos of Schisandra chinensis . Biol Plant 49, 451–454 (2005). https://doi.org/10.1007/s10535-005-0027-4

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  • DOI: https://doi.org/10.1007/s10535-005-0027-4

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