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Influence of 5′-azacitidine on promoting recovery of cell competence for shoot organogenesis in Arabidopsis

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Abstract

In Arabidopsis, adventitious shoots are formed at a high frequency when the calli are induced from roots or hypocotyls cultured on callus induction medium (CIM) and then transferred to shoot induction medium (SIM). The prolonged duration of culture on CIM decreased the frequency of shoot regeneration. However, when 5′-azacitidine (AzaC), an inhibitor of DNA methylation, was added to CIM, the excess culturing on CIM did not decrease the frequency of shoot regeneration. The level of methyl cytosine was up-regulated when hypocotyl explants were cultured on CIM for 2 weeks. We examined the expression patterns of genes that are involved in the formation or regeneration of shoots. Prolonged duration of culture on CIM up-regulated the CUC1, CLV1, CLV3, ESR1, and WUS mRNA levels, and the addition of AzaC to CIM reduced their expression levels. Our results suggest that an increase in DNA methylation decreased the shoot-forming ability and that AzaC can partially recover this ability.

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Authors and Affiliations

  1. Department of Agricultural and Life Sciences, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan

    Yoshihiko Tokuji, Shou Takano, Motoki Tonomura, Sentaro Tanaka, Kadunari Igari & Taiji Watanabe

  2. Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara, Japan

    Shou Takano & Kadunari Igari

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  1. Yoshihiko Tokuji
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  2. Shou Takano
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  3. Motoki Tonomura
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  4. Sentaro Tanaka
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  5. Kadunari Igari
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  6. Taiji Watanabe
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Correspondence to Yoshihiko Tokuji.

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Tokuji, Y., Takano, S., Tonomura, M. et al. Influence of 5′-azacitidine on promoting recovery of cell competence for shoot organogenesis in Arabidopsis . Plant Cell Tiss Organ Cult 106, 289–297 (2011). https://doi.org/10.1007/s11240-011-9920-z

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  • DOI: https://doi.org/10.1007/s11240-011-9920-z

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