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MiniMax, a new diminutive Glycine max genotype with a rapid life cycle, embryogenic potential and transformation capabilities

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Abstract

We developed Glycine max cv MiniMax (PI643148) that has a rapid life cycle, short stature and characteristic simple sequence repeat (SSR) markers that could make it useful for mutant screening, functional genomics, genetic mapping and other studies involving soybeans. We demonstrate that MiniMax is able to make somatic embryos (SEs) that rapidly develop into plantlets. Thus, the rapid cycling habit carries over into aspects of plant regeneration. Chimaeras (having transformed roots with untransformed aerial stocks) have been produced rapidly under non-axenic conditions using Agrobacterium rhizogenes-mediated transformation. Part of these experiments involved the engineering an enhanced green fluorescent protein (eGFP) reporter cassette outside the multi-cloning site of a plant expression vector, permitting non-invasive visual screening of the transformed roots. The rapid cycling growth habit of MiniMax, its ability to efficiently generate SEs and ability to be transformed should prove useful for basic aspects of G. max molecular and genetic research.

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Abbreviations

MG:

Maturity group

SCN:

Soybean cyst nematode

SSR:

Simple sequence repeat

GUS:

β-glucuronidase

eGFP:

Enhanced green fluorescent protein

FMV sgt:

Figwort mosaic virus sub-genomic transcript

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Acknowledgements

The authors thank Prakash Arelli, USDA-ARS-MSA, Crop Genetics and Production Research Unit, 605 Airways Blvd., Jackson, TN, 38301 for the H. glycines race studies in G. max cv MiniMax. The authors thank Hunter Beard for excellent technical support. We also thank Dr. Wayne Parrott (University of Georgia) for technical assistance during the development of the plant transformation procedures. This work was supported by the United Soybean Board under grant 5214. Mention of trade names or commercial products in this article is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the United States Department of Agriculture.

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Authors and Affiliations

  1. United States Department of Agriculture, Soybean Genomics and Improvement Laboratory, 10300 Baltimore Ave., BLDG 006, Beltsville, MD, 20705, USA

    Vincent P. Klink, Margaret H. MacDonald, Veronica E. Martins & Benjamin F. Matthews

  2. Molecular Physiology and Biochemistry Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, 441-100, South Korea

    Soo-Chul Park

  3. Cell and Genetics Division, National Institute of Agricultural Biotechnology, Rural Development Administration, Suwon, 441-100, South Korea

    Kyung-Hwan Kim

  4. Honam Agricultural Research Institute, NICS, RDA, Iksan, 570-080, South Korea

    So-Hyeon Baek

Authors
  1. Vincent P. Klink
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  2. Margaret H. MacDonald
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  3. Veronica E. Martins
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  4. Soo-Chul Park
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  5. Kyung-Hwan Kim
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  6. So-Hyeon Baek
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  7. Benjamin F. Matthews
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Correspondence to Vincent P. Klink.

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Klink, V.P., MacDonald, M.H., Martins, V.E. et al. MiniMax, a new diminutive Glycine max genotype with a rapid life cycle, embryogenic potential and transformation capabilities. Plant Cell Tiss Organ Cult 92, 183–195 (2008). https://doi.org/10.1007/s11240-007-9323-3

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