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Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan

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Abstract

The improvement of the induction rate in Citrus anther culture is important for taking practical advantage of the haploid potential in breeding. The influence of polyamines on anther culture of Citrus clementina, cv Nules, with particular attention to the free, soluble and insoluble-conjugated polyamine levels, has been investigated. Putrescine, spermidine and putrescine plus spermidine, were added to the standard induction medium. Before culture, spermidine was the most abundant among the free polyamines detected in anthers. The exogenous supply of either putrescine or spermidine, either independently or combined, effected greater uptake and accumulation of polyamines. The addition of 2 mM spermidine to the medium stimulated gametic embryogenesis in clementine Nules, whereas putrescine did not influence embryo production. Regenerants were mostly tri-haploids; a few doubled-haploids and no haploid plants were obtained.

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Abbreviations

DAP:

Diamino propane

PAs:

Polyamines

PCA:

Perchloric acid

PGI-1:

Phosphoglucoisomerase

PGM:

Phosphoglucomutase

PUT:

Putrescine

SNK’s test:

Student–Newman–Keuls’s test

SPD:

Spermidine

SPM:

Spermine

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Acknowledgements

The authors wish to thank Monika Hofer, Luis Navarro and Oscar Olivares-Fuster for the flow cytometry analyses and Dr. Andrew Carter (Institute of Food Research, Norwich, UK) for the English revision. This work was partially supported by the Italian PRIN 2004 to Maria Antonietta Germanà and by the fellowships of the L’Oréal “for Women in Science” 2004 to Benedetta Chiancone and Annalisa Tassoni.

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Authors and Affiliations

  1. Dipartimento SENFIMIZO Sez. Frutticoltura Mediterranea, Tropicale e Subtropicale, Facoltà di Agraria, Università degli Studi di Palermo, Viale delle Scienze 11, 90128, Palermo, Italy

    Benedetta Chiancone & Maria Antonietta Germanà

  2. Dipartimento di Biologia, Facoltà di Scienze, Università degli Studi di Bologna, Via Irnerio 42, 40126, Bologna, Italy

    Annalisa Tassoni & Nello Bagni

Authors
  1. Benedetta Chiancone
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  2. Annalisa Tassoni
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  3. Nello Bagni
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  4. Maria Antonietta Germanà
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Correspondence to Maria Antonietta Germanà.

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Chiancone, B., Tassoni, A., Bagni, N. et al. Effect of polyamines on in vitro anther culture of Citrus clementina Hort. ex Tan. Plant Cell Tiss Organ Cult 87, 145–153 (2006). https://doi.org/10.1007/s11240-006-9149-4

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  • DOI: https://doi.org/10.1007/s11240-006-9149-4

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