Abstract
Embryoids were differentiated from anthers of C. aurantifolia which were first floated on a modified Murashige and Skoog's liquid medium supplemented with 0.5 mg/l N6-benzylaminopurine and 1 mg/l indole-3-acetic acid for 20–30 d, followed by 30 d culture in semisolid Schenk and Hildebrandt's medium having the same growth hormones. Embryoids originated from within the anther lobes. Initially, a few embryoids were formed by each anther; later, they multiplied rapidly by the production of new embryoids from the hypocotyl and cotyledon portions of the original embryoids. The embryoids could develop into plantlets, which were all diploid (2n=18). The androgenic plants grew normally in soil.
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Abbreviations
- BAP:
-
N6-benzylaminopurine
- IAA:
-
indole-3-acetic acid
- MS:
-
medium of Murashige and Skoog (1962)
- SH:
-
medium of Schenk and Hildebrandt (1972)
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NBRI Research Publication No. 245 (N.S.)
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Chaturvedi, H.C., Sharma, A.K. Androgenesis in Citrus aurantifolia (Christm.) swingle. Planta 165, 142–144 (1985). https://doi.org/10.1007/BF00392223
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DOI: https://doi.org/10.1007/BF00392223