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Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration

  • Cell Biology and Morphogenesis
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Abstract

An improvement of the protocol for haploid induction through anther culture of Citrus clementina Hort. ex Tan. cv. Nules was achieved following the evaluation of a number of the factors affecting androgenesis. The influence of thidiazuron (TDZ) and three temperature pre-treatments (4°C, 25°C, 32°C) on the floral buds with respect to anther culture of C. clementina Hort. ex Tan., cv. Nules was investigated. An increased embryoid production was induced in the medium supplemented with TDZ. Pre-treatment temperatures of 4°C and 25°C were more favorable for embryo production than 32°C. Regeneration of androgenic haploid plantlets from cv. SRA 63 of C. clementina is reported here for the first time.

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Abbreviations

6-BA :

6-Benzylaminopurine

2,4-D :

2,4-Dichlorophenoxyacetic acid

GA 3 :

Gibberellic acid

KI :

Kinetin

NAA :

α-Naphthaleneacetic acid

TDZ :

Thidiazuron (N-phenyl-1,2,3,-thi-diazol-5-ylurea)

ZEA :

Zeatin

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Acknowledgements

The authors wish to thank Prof. K. Glimelius for his critical reading of the manuscript and Prof. H. Lorz for his helpful suggestions. This investigation was supported by the National Project 'Plant Biotechnology' of MiPAF.

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Authors and Affiliations

  1. Dipartimento di Colture Arboree, Facoltà di Agraria, Viale delle Scienze 11, 90128, Palermo, Italy

    M. A. Germanà & B. Chiancone

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  1. M. A. Germanà
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  2. B. Chiancone
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Correspondence to M. A. Germanà.

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Communicated by L. Peña

Both authors have contributed equally to this article.

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Germanà, M.A., Chiancone, B. Improvement of Citrus clementina Hort. ex Tan. microspore-derived embryoid induction and regeneration. Plant Cell Rep 22, 181–187 (2003). https://doi.org/10.1007/s00299-003-0669-7

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  • DOI: https://doi.org/10.1007/s00299-003-0669-7

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