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Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha

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Summary

The fusion of Citrus sinensis cv. Hamlin (sweet orange) protoplasts isolated from an embryogenic suspension culture with Severinia disticha (Philippine box orange) protoplasts isolated from epicotyl-derived callus with organogenic potential, resulted in the regeneration of allotetraploid somatic hybrid plants. Plant regeneration was a function of complementation, combining the capacity for somatic embryogenesis of C. sinensis with the organogenic ability of S. disticha. Confirmation of somatic hybrid identity was based on leaf morphology, chromosome number, and analyses of phosphoglucose mutase (PGM) and malate dehydrogenase (MDH) zymograms. Hybrid plants were multiplied organogenically and exhibited morphology intermediate to that of the parents. This is the first example of somatic hybrid plants produced between sexually incompatible woody genera.

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Authors and Affiliations

  1. Department of Fruit Crops, University of Florida, IFAS, Citrus Research and Education Center, 700 Experiment Station Road, 33850, Lake Alfred, FL, USA

    J. W. Grosser, F. G. Gmitter Jr. & J. L. Chandler

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  1. J. W. Grosser
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  2. F. G. Gmitter Jr.
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  3. J. L. Chandler
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Communicated by P. Maliga

Florida Agricultural Experiment Station Journal Series No. 8198

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Grosser, J.W., Gmitter, F.G. & Chandler, J.L. Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha . Theoret. Appl. Genetics 75, 397–401 (1988). https://doi.org/10.1007/BF00276741

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  • DOI: https://doi.org/10.1007/BF00276741

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