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Proteomic Analysis of Heat Stress Response in Leaves of Radish (Raphanus sativus L.)

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Abstract

High temperature is one of the most important abiotic factors influencing plant growth and development. Radish is a cool season vegetable crop sensitive to higher temperatures. Heat injuries may affect plant growth, and interfere with formation and thickening of the fleshy taproot. To characterize the heat-stress response in radish, 30-day-old radish seedlings were exposed to a temperature of 40 °C. Leaf samples were then collected from the seedlings at 0 h, 12 h and 24 h after temperature exposure. Proteins extracted from leaves were analyzed with two-dimensional electrophoresis (2-DE), and differentially expressed protein spots were identified by mass spectrometry (MS). In total, 11 differentially expressed proteins were identified successfully by MALDI-TOF MS. Of these, four were heat shock proteins (HSPs), four were related to energy and metabolism, two were related to redox homeostasis, and one was related to signal transduction. These proteins were analyzed further for mRNA levels, corresponding to differential levels of gene expression. The result showed that gene expression profiles at the transcriptional level were not completely consistent with those at translational levels. The differentially expressed heat stress response proteins identified, like small heat shock proteins together with energy and metabolism-related proteins, provide new insights into the molecular basis of plant responses to high temperature stresses in radish.

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Acknowledgments

This work was supported in part by grants from the Program for NCET, MOE (NCET-10-047), the National Key Technologies R&D Program of China (2012BAD02B01), the National Natural Science Foundation of China (31171956), Key Technology R&D Program of Jiangsu Province (BE2010328) and the Priority Academic Program Development of Jiangsu Province (PAPD). We thank Dr. Mohamed Ibrahim at North Dakota State University, for his critical review and helpful comments during the preparation of this manuscript.

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetics and Germplasm Enhancement; Key Laboratory of Horticultural Crop Biology and Genetic Improvement (East China), Ministry of Agriculture; College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, People’s Republic of China

    Yanyu Zhang, Liang Xu, Yiqin Gong, Fei Xiang, Xiaochuan Sun & Liwang Liu

  2. Department of Plant Sciences, North Dakota State University, Fargo, ND, 58105, USA

    Xianwen Zhu

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  1. Yanyu Zhang
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  2. Liang Xu
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Correspondence to Yiqin Gong or Liwang Liu.

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Yanyu Zhang and Liang Xu contributed equally to this work

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Zhang, Y., Xu, L., Zhu, X. et al. Proteomic Analysis of Heat Stress Response in Leaves of Radish (Raphanus sativus L.). Plant Mol Biol Rep 31, 195–203 (2013). https://doi.org/10.1007/s11105-012-0486-7

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