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Constitutive expression of mustard annexin, AnnBj1 enhances abiotic stress tolerance and fiber quality in cotton under stress

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Abstract

Annexins belong to a multigene family of Ca2+ dependent, phospholipid and cytoskeleton binding proteins. They have been shown to be upregulated under various stress conditions. We generated transgenic cotton plants expressing mustard annexin (AnnBj1), which showed enhanced tolerance towards different abiotic stress treatments like sodium chloride, mannitol, polyethylene glycol and hydrogen peroxide. The tolerance to these treatments was associated with decreased hydrogen peroxide levels and enhanced total peroxidase activity, enhanced content of osmoprotectants- proline and sucrose in transgenic plants. They showed higher retention of total chlorophyll and reduced TBARS in leaf disc assays with stress treatments, and decreased hydrogen peroxide accumulation in the stomatal guard cells when compared to their wild type counterparts. They also showed significantly enhanced fresh weight, relative water content, dry weight under stress. Treatment with sodium chloride resulted in enhanced expression of genes for ∆-pyrroline-5-carboxylase synthetase in leaves, and sucrose phosphate synthase, sucrose synthase and cellulose synthase A in the leaves and fibers of transgenic plants. The transgenic plants maintained normal seed development, fiber quality and cellulose content under stress.

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Acknowledgments

The authors are grateful to the Andhra Pradesh-Netherlands Biotechnology Programme administered by the Institute of Public Enterprise, Osmania University Campus, Hyderabad for supporting the Research work. The Research facilities under DST-FIST-II, UGC-CAS, COSIST, made available by the Head, Department of Plant Sciences are acknowledged. DK and JSK are grateful to the CSIR, Government of India for Research Fellowships.

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  1. Department of Plant Sciences, University of Hyderabad, Hyderabad, 500046, India

    Kesanakurti Divya, S. K. Jami & P. B. Kirti

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  1. Kesanakurti Divya
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  2. S. K. Jami
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  3. P. B. Kirti
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Correspondence to P. B. Kirti.

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Divya, K., Jami, S.K. & Kirti, P.B. Constitutive expression of mustard annexin, AnnBj1 enhances abiotic stress tolerance and fiber quality in cotton under stress. Plant Mol Biol 73, 293–308 (2010). https://doi.org/10.1007/s11103-010-9615-6

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