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Proteomic analysis of early germs with high-oil and normal inbred lines in maize

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Abstract

High-oil maize as a product of long-term selection provides a unique resource for functional genomics. In this study, the abundant soluble proteins of early developing germs from high-oil and normal lines of maize were compared using two-dimensional gel electrophoresis (2-DGE) in combination with mass spectrometry (MS). More than 1100 protein spots were detected on electrophoresis maps of both high-oil and normal lines by using silver staining method. A total of 83 protein spots showed significant differential expression (>two-fold change; t-test: P < 0.05) between high-oil and normal inbred lines. Twenty-seven protein spots including 25 non-redundant proteins were identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF-MS). Functional categorization of these proteins was carbohydrate metabolism, cytoskeleton, protein metabolism, stress response, and lipid metabolism. Three such proteins involved in lipid metabolism, namely putative enoyl-ACP reductase (ENR), putative stearoyl-ACP desaturase (SAD) and putative acetyl-CoA C-acyltransferase (ACA), had more abundant expressions in high-oil lines than in normal. At the mRNA expression level, SAD, ENR and ACA were expressed at significantly higher levels in high-oil lines than in normal. The results demonstrated that high expressions of SAD, ENR and ACA might be associated to increasing oil concentration in high-oil maize. This study represents the first proteomic analysis of high-oil maize and contributes to a better understanding of the molecular basis of oil accumulation in high-oil maize.

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Acknowledgements

We thank the National Nature Science Foundation of China (30571165) and Agriculture Ministry of China (2004-Z25) for funding.

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Authors and Affiliations

  1. National Maize Improvement Center of China, China Agricultural University, Beijing, 100094, China

    Zhanji Liu, Xiaohong Yang, Yang Fu, Yirong Zhang, Jianbin Yan, Tongming Song & Jiansheng Li

  2. Hi-Tech Research Centre, Shandong Academy of Agricultural Sciences, Jinan, 250100, China

    Zhanji Liu

  3. Department of Crop Sciences, University of Illinois, 1102 S. Goodwin Ave, Urbana, IL, 61801, USA

    T. Rocheford

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  1. Zhanji Liu
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  2. Xiaohong Yang
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Correspondence to Jiansheng Li.

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Liu, Z., Yang, X., Fu, Y. et al. Proteomic analysis of early germs with high-oil and normal inbred lines in maize. Mol Biol Rep 36, 813–821 (2009). https://doi.org/10.1007/s11033-008-9250-3

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