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Oxidative stress is associated with aluminum toxicity recovery in apex of pea root

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Abstract

Aims

Although many studies on the mechanism of Al toxicity and tolerance have been conducted independently, events occurring during the recovery process from Al injury is limited. This study was to investigate Al toxicity recovery mechanism focusing in morphological and physiological aspect.

Methods

We investigated the mechanisms underlying Al toxicity recovery in terms of oxidative stress using the pea root apex as a model system.

Results

The accumulation of reactive oxygen species was remarkably high in the root under continued Al treatment but decreased in the recovering root. The superoxide anion exuded in the presence of nicotinamide adenine dinucleotide phosphate (NADPH) showed a similar tendency with respect to the accumulation of reactive oxygen species. A similar pattern of lignin content and superoxide dismutase activity was observed among the treatments, while the increased peroxidation in the root under continued Al treatment did not decline with recovery treatment. A longitudinal section of the root under continued Al treatment showed the accumulation of superoxide anion, lignin and peroxide (H2O2) at the epidermal and outer cortex region where the Al induced injuries, including ruptures, are detected.

Conclusions

Oxidative stress is associated with the mechanism of Al toxicity recovery. The recovery process might include the elongation of the central cylinder as a consequence of the oxidative stress-induced formation of the zonal region (ZR). The results further suggest a plausible role for the ZR in the programmed cell death-like function involved in Al toxicity recovery.

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Acknowledgements

This research was financially supported through a grant from the Japanese Ministry of Education, Sports, Science and Technology to H.M [Grand-in-Aid for Scientific Research C (2258007)].

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Authors and Affiliations

  1. Research Institute of Health and Welfare, KIBI International University, 8 Iga-machi, Takahashi-city, Okayama, 716-8508, Japan

    Hideaki Matsumoto & Hirotoshi Motoda

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  1. Hideaki Matsumoto
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  2. Hirotoshi Motoda
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Correspondence to Hideaki Matsumoto.

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Responsible Editor: Juan Barceló.

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Matsumoto, H., Motoda, H. Oxidative stress is associated with aluminum toxicity recovery in apex of pea root. Plant Soil 363, 399–410 (2013). https://doi.org/10.1007/s11104-012-1396-z

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