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H2O2 production and antioxidant responses in seeds and early seedlings of two different rice varieties exposed to aluminum

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Abstract

The effect of Al stress on H2O2 production of rice (Oryza sativa L.) seedlings and difference in responses of antioxidant enzymes between Al-tolerant variety (Azucena) and Al-sensitive rice one (IR 64) were investigated. Aluminum-induced H2O2 production and malondialdehyde (MDA) content were more pronounced for IR 64 than for Azucena. In the presence of 2 mM Al, addition of 10 mM imidazole (inhibitor of NADPH oxidase) and 1 mM azide (inhibitor of peroxidase) significantly decreased H2O2 production by 16% and 43% for Azucena, and 21% and 68% for IR 64, respectively. Under Al treatment, the Al-tolerant variety Azucena had significantly higher activities of catalase, ascorbate peroxidase, dehydroascorbate reducase, glutathione peroxidase and glutathione reductase, and higher concentrations of reduced glutathione than the Al-sensitive one IR 64. Treatment with buthionine sulfoximine, a specific inhibitor of GSH synthesis, significantly increased H2O2 production in both varieties in the presence and absence of Al. In contrast, the treatment with GSH significantly decreased the production of H2O2 induced by Al stress. Results suggest that GSH may play an important role in scavenging H2O2 caused by Al stress.

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Abbreviations

Al:

Aluminum

APX:

Ascorbate peroxidase

BSO:

Buthionine sulfoximine

CAT:

Catalase

DHAR:

Dehydroascorbate reducase

GPX:

Glutathione peroxidase

GR:

Glutathione reductase

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

H2O2 :

Hydrogen peroxide

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgements

This research project was supported by the National Natural Science Foundation of China (No. 30228023) and the Teaching and Research Award Program for Outstanding Young Teachers in Higher Education Institutions of MOE.

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, P. R. China

    Baohui Ma & Zhenguo Shen

  2. National Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing, 210095, P. R. China

    Jianmin Wan

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  1. Baohui Ma
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  2. Jianmin Wan
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Correspondence to Zhenguo Shen.

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Ma, B., Wan, J. & Shen, Z. H2O2 production and antioxidant responses in seeds and early seedlings of two different rice varieties exposed to aluminum. Plant Growth Regul 52, 91–100 (2007). https://doi.org/10.1007/s10725-007-9183-1

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  • DOI: https://doi.org/10.1007/s10725-007-9183-1

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