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Self-compatible peach (Prunus persica) has mutant versions of the S haplotypes found in self-incompatible Prunus species

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Abstract

This study demonstrates that self-compatible (SC) peach has mutant versions of S haplotypes that are present in self-incompatible (SI) Prunus species. All three peach S haplotypes, S 1, S 2, and S 2m, found in this study encode mutated pollen determinants, SFB, while only S 2m has a mutation that affects the function of the pistil determinant S-RNase. A cysteine residue in the C5 domain of the S 2m-RNase is substituted by a tyrosine residue, thereby reducing RNase stability. The peach SFB mutations are similar to the SFB mutations found in SC haplotypes of sweet cherry (P. avium) and Japanese apricot (P. mume). SFB 1 of the S 1 haplotype, a mutant version of almond (P. dulcis) S k haplotype, encodes truncated SFB due to a 155 bp insertion. SFB 2 of the S 2 and S 2m haplotypes, both of which are mutant versions of the S a haplotype in Japanese plum (P. salicina), encodes a truncated SFB due to a 5 bp insertion. Thus, regardless of the functionality of the pistil determinant, all three peach S haplotypes are SC haplotypes. Our finding that peach has mutant versions of S haplotypes that function in almond and Japanese plum, which are phylogenetically close and remote species, respectively, to peach in the subfamily Prunoideae of the Roasaceae, provides insight into the SC/SI evolution in Prunus. We discuss the significance of SC pollen part mutation in peach with special reference to possible differences in the SI mechanisms between Prunus and Solanaceae.

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Abbreviations

GSI:

gametophytic self-incompatibility

Pollen component:

pollen S

SC:

self-compatibility

SFB :

S-haplotype-specific F-box gene

SI:

self-incompatibility

SLFL :

S locus F-box genes with the low allelic sequence polymorphism

S-RNase:

self-incompatibility RNase

Stylar component:

pistil S

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Acknowledgements

The authors gratefully acknowledge the helpful comments from Hidenori Sassa of Chiba Univ., Koichiro Ushijima of Okayama Univ., and Amy Iezzoni of Michigan State Univ., and the gifts of plant material from Yukio Sasabe at the Okayama Prefectural Agricultural Experimental Station, Okayama, Japan and Rafael Socias i Company at the Unidad de Fruticultura, Centro de Investigación y Tecnología Agroalimentaria de Aragón, Zaragosa, Spain. This work was supported by a Grant-in-Aid (no. 17380021) for Scientific Research (B) to R.T.

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Authors and Affiliations

  1. Laboratory of Pomology, Graduate School of Agriculture, Kyoto University, Kyoto , 606-8502, Japan

    Ryutaro Tao, Akiko Watari, Toshio Hanada & Hisayo Yamane

  2. Experimental Farm, Graduate School of Agriculture, Kyoto University, Takatsuki , 569-0096, Japan

    Tsuyoshi Habu

  3. National Institute of Fruit Tree Science, Tsukuba , 305-8605, Japan

    Hideaki Yaegaki & Masami Yamaguchi

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  1. Ryutaro Tao
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  2. Akiko Watari
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  7. Hisayo Yamane
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Correspondence to Ryutaro Tao.

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Tao, R., Watari, A., Hanada, T. et al. Self-compatible peach (Prunus persica) has mutant versions of the S haplotypes found in self-incompatible Prunus species. Plant Mol Biol 63, 109–123 (2007). https://doi.org/10.1007/s11103-006-9076-0

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