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Genetic and molecular analysis in Cristobalina sweet cherry, a spontaneous self-compatible mutant

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Abstract

Self-compatibility in a naturally self-incompatible species like sweet cherry is a highly interesting trait for breeding purposes and a powerful tool with which to investigate the basis of the self-incompatible reaction in gametophytic systems. However, natural self-compatibility in sweet cherry is a very rare phenomenon. Cristobalina is a local Spanish sweet cherry cultivar that has proven to be spontaneously self-compatible. In this work, the nature of the self-compatibility in Cristobalina has been studied using genetic and molecular approaches. Pollination studies and microscopic observations of pollen tube growth were carried out to confirm the self-compatible character and the results obtained indicate that self-compatibility is caused by a failure of the pollen and not the style factor. Polymerase chain reaction (PCR) analysis of progenies derived from Cristobalina revealed that self-compatibility in this genotype is not related uniquely to one of the two pollen S alleles, but that pollen grains carrying either of the two haplotypes can overcome the incompatibility barrier. Moreover, PCR analysis and microscopic observation of pollen tube growth in progeny derived from Cristobalina also confirmed that the self-compatible descendants can carry either of the two S haplotypes of their progenitor. Isolation and sequencing of the style S-RNases and pollen SFBs revealed that the DNA sequences of these factors are the same as those described in other self-incompatible sweet cherry cultivars with the same S alleles. Possible mechanisms to explain self-compatibility in Cristobalina are discussed.

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Acknowledgements

We gratefully acknowledge M. Herrero and J. Rodrigo for helpful comments on the manuscript and J. Negueroles for providing some of the plant material used in this study. Financial support for this work was provided by the Spanish Ministry of Science and Technology (MCYT Project grant AGL2001–2414 and AGL2003–05318). A. W. was supported by a SIA-DGA fellowship.

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Authors and Affiliations

  1. CITA de Aragón, Campus de Aula Dei, Apdo. 727, Zaragoza, 50080, Spain

    A. Wünsch

  2. Estación Experimental la Mayora – CSIC, 29750 Algarrobo-Costa, Málaga, Spain

    J. I. Hormaza

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  1. A. Wünsch
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  2. J. I. Hormaza
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Correspondence to J. I. Hormaza.

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Wünsch, A., Hormaza, J.I. Genetic and molecular analysis in Cristobalina sweet cherry, a spontaneous self-compatible mutant. Sex Plant Reprod 17, 203–210 (2004). https://doi.org/10.1007/s00497-004-0234-8

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