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Effects of 5-aminolevulinic acid treatment on photosynthesis of strawberry

  • Original Paper
  • Published:
Photosynthetica

Abstract

Effects of root treatment with 5-aminolevulinic acid (ALA) on leaf photosynthesis in strawberry (Fragaria ananassa Duch.) plants were investigated by rapid chlorophyll fluorescence and modulated 820 nm reflection using 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU) and methyl viologen (MV). Our results showed that ALA treatments increased the net photosynthetic rate and decreased the intercelluar CO2 concentration in strawberry leaves. Under DCMU treatment, trapping energy for QA reduction per PSII reaction center increased greatly, indicating DCMU inhibited electron transfer from QA −. The maximum photochemical efficiency of PSII (Fv/Fm) decreased under the DCMU treatment, while a higher Fv/Fm remained in the ALA-pretreated plants. Not only the parameters related to a photochemical phase, but also that one related to a heat phase remained lower after the ALA pretreatment, compared to the sole DCMU treatment. The MV treatment decreased PSI photochemical capacity. The results of modulated 820 nm reflection analysis showed that DCMU and MV treatments had low re-reduction of P700 and plastocyanin (PSI). However, the strawberry leaf discs pretreated with ALA exhibited high re-reduction of PSI under DCMU and MV treatments. The results of this study suggest that the improvement of photosynthesis by ALA in strawberry was not only related to PSII, but also to PSI and electron transfer chain.

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Abbreviations

ALA:

5-aminolevulinic acid

C i :

intercellular CO2 concentration

Chl:

chlorophyll

DCMU:

3-(3,4-dichlorophenyl)-1,1-dimethyl urea

E :

transpiration rate

Fj and Fi :

the fluorescence intensity at 2 ms (J-step) and 30 ms (I-step)

Fm :

the maximum fluorescence intensity

Fo :

the minimum fluorescence intensity

Fv/Fm :

maximal quantum yield of PSII photochemistry

g s :

stomatal conductance

MR:

modulated 820 nm reflection

MRo :

value of modulated 820 nm reflection at the onset of red light illumination

MV:

methyl viologen

P680:

PSII reaction center

P700:

PSI reaction center

P N :

net photosynthetic rate

PC:

plastocyanin

PQH2 :

plastoquinol

RC/ABS:

QA reducing reaction centers per PSII antenna chlorophyll

VJ :

the relative variable fluorescence intensities at the J-step

VI :

the relative variable fluorescence intensities at the I-step

VPSI :

maximum slope of decrease of MR/MRo

VPSII-PSI :

maximum slope of increase of MR/MRo

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Authors and Affiliations

  1. Institute of Nanjing Agricultural Sciences, Jiangsu Academy of Agricultural Sciences, Nanjing, 210046, China

    Y. P. Sun, R. X. Cao & C. B. Guo

  2. Department of Biological and Environmental Sciences, University of Hertfordshire, Hatfield, AL10 9AB, UK

    Y. P. Sun, Y. J. Huang & A. M. Hall

  3. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    J. Liu & L. J. Wang

Authors
  1. Y. P. Sun
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  2. J. Liu
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  3. R. X. Cao
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  4. Y. J. Huang
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  5. A. M. Hall
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  6. C. B. Guo
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  7. L. J. Wang
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Corresponding authors

Correspondence to C. B. Guo or L. J. Wang.

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Acknowledgements: This research was supported by Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF), CX(11)4004, China.

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Sun, Y.P., Liu, J., Cao, R.X. et al. Effects of 5-aminolevulinic acid treatment on photosynthesis of strawberry. Photosynthetica 55, 276–284 (2017). https://doi.org/10.1007/s11099-016-0667-y

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  • DOI: https://doi.org/10.1007/s11099-016-0667-y

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