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Submergence effects on rice genotypes during seedling stage: Probing of submergence driven changes of photosystem 2 by chlorophyll a fluorescence induction O-J-I-P transients

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Photosynthetica

Abstract

The effects of submergence on chlorophyll (Chl) a fluorescence were compared in seven Oryza sativa (L.) cultivars, namely FR 13A, Khoda, Khadara, Kalaputia (tolerant), Sabita, and Hatipanjari (avoiding type), and IR 42 (susceptible). Seedlings were submerged for 4 d under complete darkness. Oxygen concentration of flood water decreased with the period of submergence with concomitant increase in concentration of carbon dioxide. Submergence caused diminution in the amount of total Chl. Genotypic differences were observed for Chl content and survival percentage. Quantification of the Chl a fluorescence transients (JIP-test) revealed large cultivar differences in the response of photosystem 2 (PS2) to submergence. The kinetics of Chl a fluorescence rise showed complex changes in the magnitudes and rise of O-J, J-I, and I-P phases caused by submergence. The selective suppression of the J-I phase of fluorescence especially after 2 d of submergence provided evidence for weakened electron donation from the oxygen evolving complex whereas under severe submergence stress (4 d) both O-J and J-I steps were suppressed greatly with highly suppressed P-step, which resulted in lowering of variable fluorescence. Grouping probability or energetic connectivity between PS2 obtained through JIP-test from the data after 2 d of submergence showed a direct relation with survival percentage, i.e. fluorescence measurements contained the information of the survival chance of a plant under submerged conditions. The information could be used in identifying the submergence tolerant cultivars when the damage is not very severe.

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Abbreviations

area:

the space above the fluorescence curve between F0 and Fm

Chl:

chlorophyll

DAS:

days after submergence

DI0/CS:

dissipation of energy in a PS2 cross-section

ET0/CS:

electron transport in a PS2 cross-section

F0 :

minimal fluorescence

Fm :

maximal fluorescence

Fv (= Fm − F0):

variable fluorescence

Fv/Fm :

maximum photochemical efficiency of PS2

F50µs, F300µs, and F2ms :

fluorescence at 50 or 300 µs, and 2 ms, respectively

OEC:

oxygen evolving complex

PG:

grouping probability

PIABS :

performance index on the basis of utilization of absorbed energy

PQ:

plastoquinone

PS:

photosystem

QA :

primary electron acceptor of PS2

QB :

secondary electron acceptor of PS2

RC/CS0 :

number of reaction centres per excited cross-section

VJ :

(F2ms − F0)/(Fm − F0)

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Authors and Affiliations

  1. Division of Biochemistry, Plant Physiology & Environmental Sciences, Central Rice Research Institute, Cuttack, Orissa, 753 006, India

    D. Panda, D. N. Rao, S. G. Sharma & R. K. Sarkar

  2. Bioenergetics Laboratory, University of Geneva, CH-1254, Jussy-Geneva, Switzerland

    R. J. Strasser

Authors
  1. D. Panda
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  2. D. N. Rao
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  3. S. G. Sharma
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  4. R. J. Strasser
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  5. R. K. Sarkar
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Correspondence to R. K. Sarkar.

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Panda, D., Rao, D.N., Sharma, S.G. et al. Submergence effects on rice genotypes during seedling stage: Probing of submergence driven changes of photosystem 2 by chlorophyll a fluorescence induction O-J-I-P transients. Photosynthetica 44, 69–75 (2006). https://doi.org/10.1007/s11099-005-0200-1

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  • DOI: https://doi.org/10.1007/s11099-005-0200-1

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