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Acclimation of hydrogen peroxide enhances salt tolerance by activating defense-related proteins in Panax ginseng C.A. Meyer

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Abstract

The effect of exogenously applied hydrogen peroxide on salt stress tolerance was investigated in Panax ginseng. Pretreatment of ginseng seedlings with 100 μM H2O2 increased the physiological salt tolerance of the ginseng plant and was used as the optimum concentration to induce salt tolerance capacity. Treatment with exogenous H2O2 for 2 days significantly enhanced salt stress tolerance in ginseng seedlings by increasing the activities of ascorbate peroxidase, catalase and guaiacol peroxidase and by decreasing the concentrations of malondialdehyde (MDA) and endogenous H2O2 as well as the production rate of superoxide radical (O2 −). There was a positive physiological effect on the growth and development of salt-stressed seedlings by exogenous H2O2 as measured by ginseng dry weight and both chlorophyll and carotenoid contents. Exogenous H2O2 induced changes in MDA, O2 −, antioxidant enzymes and antioxidant compounds, which are responsible for increases in salt stress tolerance. Salt treatment caused drastic declines in ginseng growth and antioxidants levels; whereas, acclimation treatment with H2O2 allowed the ginseng seedlings to recover from salt stress by up-regulation of defense-related proteins such as antioxidant enzymes and antioxidant compounds.

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Acknowledgments

This research was supported by iPET (112142-05-1-CG000), Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries, Republic of Korea.

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Authors and Affiliations

  1. Korean Ginseng Center for Most Valuable Products & Ginseng Genetic Resource Bank, Kyung Hee University, Suwon, 449-701, South Korea

    Gayathri Sathiyaraj, Sathiyaraj Srinivasan, Yu-Jin Kim, Ok Ran Lee, Shonana Parvin, Sri Renuka Devi Balusamy, Atlanzul Khorolragchaa & Deok Chun Yang

  2. Department of Oriental Medicinal Material & Processing, College of Life Science, Kyung Hee University, 1 Seocheon, Kiheung Yongin, Kyunggi, 449-701, South Korea

    Deok Chun Yang

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  1. Gayathri Sathiyaraj
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  2. Sathiyaraj Srinivasan
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  3. Yu-Jin Kim
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  4. Ok Ran Lee
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  5. Shonana Parvin
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  6. Sri Renuka Devi Balusamy
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  7. Atlanzul Khorolragchaa
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Correspondence to Deok Chun Yang.

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Gayathri Sathiyaraj and Sathiyaraj Srinivasan contributed equally to this work.

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Sathiyaraj, G., Srinivasan, S., Kim, YJ. et al. Acclimation of hydrogen peroxide enhances salt tolerance by activating defense-related proteins in Panax ginseng C.A. Meyer. Mol Biol Rep 41, 3761–3771 (2014). https://doi.org/10.1007/s11033-014-3241-3

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