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Comparative analysis of genetic diversity in sacred lotus (Nelumbo nucifera Gaertn.) using AFLP and SSR markers

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Abstract

The sacred lotus (Nelumbo nucifera Gaertn.) is an aquatic plant of economic and ornamental importance in China. In this study, we developed twenty novel sacred lotus SSR markers, and used AFLP and SSR markers to investigate the genetic diversity and genetic relationships among 58 accessions of N. nucifera including 15 seed lotus, 12 rhizome lotus, 24 flower lotus and 7 wild lotus. Our results showed that sacred lotus exhibited a low level of genetic diversity, which may attribute to asexual reproduction and long-term artificial selection. A dendrogram based on both AFLP and SSR clustering data showed that: (1) the seed lotus accessions and rhizome lotus accessions were distinctly clustered into different groups, which indicated the significant genetic differentiation between them. This may be attributed to the two modes of reproduction and lack of genetic exchange; (2) the accessions of Thailand wild lotus were separated from other wild lotus accessions. This implied that the Thailand lotus might be genetically differentiated from other wild lotuses. In addition, Mantel test conducted gave highly significant correlation between AFLP-SSR data and each of the AFLP and SSR ones, with the values of r = 0.941 and r = 0.879, respectively, indicating the higher efficiency of the combination of these techniques (AFLP and SSR) in estimation and validation of the genetic diversity among the accession of sacred lotus. This knowledge of the genetic diversity and genetic relatedness of N. nucifera is potentially useful to improve the current strategies in breeding and germplasm conservation to enhance the ornamental and economic value of sacred lotus.

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Acknowledgments

We are grateful to Dr. F. James Rohlf (Department of Ecology and Evolution, Stony Brook University, USA) and Dr. Sudheer Pamidimarri (Shree M. and N. Virani Science College, Saurashtra University, India) for their help with bootstrap analysis). This work was supported by National Nature Science Foundation of China (Grant no. 30771152).

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Authors and Affiliations

  1. State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan, 430072, People’s Republic of China

    Jihong Hu, Lei Pan, Honggao Liu, Shuzhen Wang, Zhihua Wu & Yi Ding

  2. Wuhan National Germplasm Repository for Aquatic Vegetables, Wuhan, 430065, People’s Republic of China

    Weidong Ke & Yi Ding

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  1. Jihong Hu
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  2. Lei Pan
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  3. Honggao Liu
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  4. Shuzhen Wang
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  5. Zhihua Wu
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  6. Weidong Ke
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  7. Yi Ding
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Correspondence to Yi Ding.

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Hu, J., Pan, L., Liu, H. et al. Comparative analysis of genetic diversity in sacred lotus (Nelumbo nucifera Gaertn.) using AFLP and SSR markers. Mol Biol Rep 39, 3637–3647 (2012). https://doi.org/10.1007/s11033-011-1138-y

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  • DOI: https://doi.org/10.1007/s11033-011-1138-y

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