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Development and characterization of microsatellite loci for lotus (Nelumbo nucifera)

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Abstract

This paper reports the development of microsatellite primers for Nelumbo nucifera Gaerten. By screening genomic libraries enriched with 10 kinds of probes, Seventeen polymorphic loci were isolated and primers were designed. Polymorphism of these 17 loci was assessed in 24 individuals. All the 17 loci are polymorphic and the number of alleles ranged from two to seven. Observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.9176 and from 0.2837 to 0.7917 respectively. These microsatellite loci should be useful for studying the genetic diversity of N. nucifera.

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Acknowledgements

We thank our colleagues Yunjuan Zuo and Jinmei Zhang for their assistance in the experiment. The research was supported by the National Natural Science Foundation of China (30121003), Natural Science Foundation of Heilongjiang province (C2007-19) and the Aquatic Plant Research Grant awarded to Grant Mitchell by the International Waterlily and Water Gardening Society.

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Authors and Affiliations

  1. State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Xiangshan, Beijing, 100093, China

    Hong-Li Tian, Xiao-Qing Chen, Jian-Xiu Wang, Jun Wen & Shi-Liang Zhou

  2. Graduate School of the Chinese Academy of Sciences, Beijing, 100039, China

    Hong-Li Tian, Xiao-Qing Chen & Jian-Xiu Wang

  3. Department of Life Sciences, Daqing Normal College, Daqing, 163712, China

    Jian-Hua Xue

  4. Department of Botany, National Museum of Natural History, Smithsonian Institution, Washington, DC, 20013-7012, USA

    Jun Wen

  5. Liang Lian Lotus & Waterlily Farm, R. M. B. 824, Bunga, 2550, NSW, Australia

    Grant Mitchell

Authors
  1. Hong-Li Tian
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  2. Xiao-Qing Chen
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  3. Jian-Xiu Wang
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  4. Jian-Hua Xue
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  5. Jun Wen
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  6. Grant Mitchell
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  7. Shi-Liang Zhou
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Corresponding author

Correspondence to Shi-Liang Zhou.

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Tian, HL., Chen, XQ., Wang, JX. et al. Development and characterization of microsatellite loci for lotus (Nelumbo nucifera). Conserv Genet 9, 1385–1388 (2008). https://doi.org/10.1007/s10592-007-9503-z

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  • DOI: https://doi.org/10.1007/s10592-007-9503-z

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