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Identification and pyramiding of QTLs for cold tolerance at the bud bursting and the seedling stages by use of single segment substitution lines in rice (Oryza sativa L.)

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Abstract

Cold stress is one of the main constraints in rice production, and damage from cold can occur at different developmental stages in rice. Understanding the genetic basis of cold tolerance is the key for breeding cold-tolerant variety. In this study, we used single segment substitution lines (SSSLs) derived from a cross between cold-tolerant japonica variety “Nan-yang-zhan” and a popular indica variety “Hua-jing-xian 74” to detect and pyramid QTLs for cold tolerance at the bud bursting and the seedling stages. Evaluation of cold tolerance of these SSSLs and their recurrent parent helped identify two cold-tolerant QTLs (qCTBB-5 and qCTBB-6) at the bud bursting stage and two cold-tolerant QTLs (qCTS-6 and qCTS-12) at the seedling stage. The SSSLs carrying these QTLs showed stronger cold tolerance than their recurrent parent HJX74 did in three independent experiments. The qCTBB-6 and qCTS-6 were mapped to the same chromosomal region. QTL pyramiding was performed by intercrossing of SSSLs carrying the respective QTLs for cold tolerance at the bud bursting stage and the seedling stage and marker-assisted selection (MAS). The selected pyramiding line SC1-1 with different cold-tolerant QTLs showed cumulative effects on cold tolerance. Our results suggest that different genes (QTLs) control cold tolerance at bud bursting and seedling stages, and pyramiding of stable expression QTLs for cold tolerance at different developmental stages through MAS is a good strategy to prevent cold damage in rice.

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References

  • Andaya V, Mackill D (2003a) Mapping of QTLs associated with cold tolerance during the vegetative stage in rice. J Exp Bot 54:2579–2585

    Article  CAS  PubMed  Google Scholar 

  • Andaya V, Mackill D (2003b) QTLs conferring cold tolerance at the booting stage of rice using recombinant inbred lines from a japonica × indica cross. Theor Appl Genet 106:1084–1090

    CAS  PubMed  Google Scholar 

  • Dai LY, Lin XH, Ye CR, Ise K, Saito K, Kato A, Xu FR, Yu TQ, Zhang DP (2004) Identification of quantitative trait loci controlling cold tolerance at the reproductive stage in Yunnan landrace of rice, Kunmingxiaobaigu. Breed Sci 54:253–258

    Article  CAS  Google Scholar 

  • Eshed Y, Zamir D (1995) An introgression line population of Lycopersicon pennellii in the cultivated tomato enables the identification and fine mapping of yield-associated QTL. Genetics 141:1147–1162

    CAS  PubMed  PubMed Central  Google Scholar 

  • Ji SL, Jiang L, Wang YH, Liu SJ, Liu X, Zhai HQ, Wan JM (2007) Detection and analysis of QTL for germination rate at low temperature in rice (Oryza sativa L.) using backcross inbred lines. J Nanjing Agric Univ 30:1–6

    CAS  Google Scholar 

  • Kaw R, Khush GS (1986) Combining ability for low-temperature tolerance in rice. Rice Genet I:593–612

    Google Scholar 

  • Kearsey MJ, Farquhar AGL (1998) QTL analysis in plants; where are we now? Heredity 80:137–142

    Article  PubMed  Google Scholar 

  • Koseki M, Kitazawa N, Yonebayashi S, Maehara Y, Wang ZX, Minobe Y (2010) Identification and fine mapping of a major quantitative trait locus originating from wild rice, controlling cold tolerance at the seedling stage. Mol Genet Genomics 284:45–54

    Article  CAS  PubMed  Google Scholar 

  • Li ZK, Yu SB, Lafitte HR, Huang N, Courtois B, Hittalmani S, Vijayakumar CHM, Liu GF, Wang GC, Shashidhar HE, Zhuang JY, Zheng KL, Singh VP, Sidhu JS, Srivantaneeyakul S, Khush GS (2003) QTL × environment interactions in rice. I. Heading date and plant height. Theor Appl Genet 108:141–153

    Article  CAS  PubMed  Google Scholar 

  • Lou QJ, Chen L, Sun ZX, Xing YZ, Li J, Xu XY, Mei HW, Luo LJ (2007) A major QTL associated with cold tolerance at seedling stage in rice (Oryza sativa L.). Euphytica 158:87–94

    Article  CAS  Google Scholar 

  • Mao DH, Yu L, Chen DZ, Li LY, Zhu YX, Xiao YQ, Zhang DC, Chen CY (2015) Multiple cold resistance loci confer the high cold tolerance adaptation of Dongxiang wild rice (Oryza rufipogon) to its high-latitude habitat. Theor Appl Genet 128:1359–1371

    Article  CAS  PubMed  Google Scholar 

  • McCouch SR, Cho YG, Yano M, Paul E, Blinsturb M (1997) Report on QTL nomenclature. Rice Genet Newsl 14:11–13

    Google Scholar 

  • Miura K, Lin SY, Yano M, Nagamine T (2001) Mapping quantitative trait loci controlling low temperature germinability in rice (Oryza sativa L.). Breed Sci 51:293–299

    Article  CAS  Google Scholar 

  • Murray MG, Thompson WK (1980) Rapid isolation of high molecular-weight plant DNA. Nucleic Acids Res 8:4321–4325

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ranawake AL, Manangkil OE, Yoshida S, Ishii T, Mori N, Nakamura C (2014) Mapping QTLs for cold tolerance at germination and the early seedling stage in rice (Oryza sativa L.). Biotechnol Biotechnol Equip 28:989–998

    Article  PubMed  PubMed Central  Google Scholar 

  • Suh J, Jeung J, Lee J, Choi Y, Yea J, Virk P, Mackill D, Jena K (2010) Identification and analysis of QTLs controlling cold tolerance at the reproductive stage and validation of effective QTLs in cold-tolerant genotypes of rice (Oryza sativa L.). Theor Appl Genet 120:985–995

    Article  CAS  PubMed  Google Scholar 

  • Suh J, Lee C, Lee J, Kim J, Kim S, Cho Y, Park S, Shin J, Kim Y, Jena K (2012) Identification of quantitative trait loci for seedling cold tolerance using RILs derived from a cross between japonica and tropical japonica rice cultivars. Euphytica 184:101–108

    Article  Google Scholar 

  • Wang ZF, Wang FH, Zhou R, Wang JF, Zhang HS (2011) Identification of quantitative trait loci for cold tolerance during the germination and seedling stages in rice (Oryza sativa L.). Euphytica 181:405–413

    Article  Google Scholar 

  • Xi ZY, He FH, Zeng RZ, Zhang ZM, Ding XH, Li WT, Zhang GQ (2006) Development of a wide population of chromosome single-segment substitution lines in the genetic background of an elite cultivar of rice (Oryza sativa L.). Genome 49:476–484

    Article  CAS  PubMed  Google Scholar 

  • Yang TF, Zhang SH, Zhao JL, Huang ZH, Zhang GQ, Liu B (2015) Meta-analysis of QTLs underlying cold tolerance in rice (Oryza sativa L.). Mol Plant Breed 13:1–15

    Google Scholar 

  • Zhang GQ, Zeng RZ, Zhang ZM, Ding XH, Li WT, Liu GF, He FH, Tulukdar A, Huang CF, Xi ZY, Qin LJ, Shi JQ, Zhao FM, Feng MJ, Shan ZL, Chen L, Guo XQ, Zhu HT, Lu YG (2004) The construction of a library of single segment substitution lines in rice (Oryza sativa L.). Rice Genetic Newsl 121:85–87

    Google Scholar 

  • Zhang ZH, Yu SB, Yu T, Huang Z, Zhu YG (2005) Mapping quantitative trait loci (QTLs) for seedling-vigor using recombinant inbred lines of rice (Oryza sativa L.). Field Crop Res 91:161–170

    Article  Google Scholar 

  • Zhang Q, Chen QH, Wang SL, Hong YH, Wang ZL (2014a) Rice and cold stress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci. Rice 7:24

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhang SH, Zheng JS, Liu B, Peng SB, Leung H, Zhao JL, Wang XF, Yang TF, Huang ZH (2014b) Identification of QTLs for cold tolerance at seedling stage in rice (Oryza sativa L.) using two distinct methods of cold treatment. Euphytica 195:95–104

    Article  CAS  Google Scholar 

  • Zhuang JY, Lin HX, Lu J, Qian HR, Hittalmani S, Huang N, Zheng KL (1997) Analysis of QTL × environment interaction for yield components and plant height in rice. Theor Appl Genet 95:799–808

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was supported by National Natural Science Foundation of China (31501389), Guangdong Scientific and Technological Plan (2014B070706013, 2015B020201001) and the Presidential Foundation of Guangdong Academy of Agricultural Sciences, China (201422).

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Authors and Affiliations

  1. Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China

    Tifeng Yang, Shaohong Zhang, Junliang Zhao, Qing Liu, Zhanghui Huang, Xingxue Mao, Jingfang Dong, Xiaofei Wang & Bin Liu

  2. State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China

    Tifeng Yang & Guiquan Zhang

  3. Guangdong Key Laboratory of New Technology in Rice Breeding, Guangzhou, 510640, China

    Tifeng Yang, Shaohong Zhang, Junliang Zhao, Qing Liu, Zhanghui Huang, Xingxue Mao, Jingfang Dong, Xiaofei Wang & Bin Liu

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  1. Tifeng Yang
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  2. Shaohong Zhang
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  3. Junliang Zhao
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  4. Qing Liu
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  5. Zhanghui Huang
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  6. Xingxue Mao
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  7. Jingfang Dong
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  8. Xiaofei Wang
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  9. Guiquan Zhang
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  10. Bin Liu
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Correspondence to Guiquan Zhang or Bin Liu.

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Yang, T., Zhang, S., Zhao, J. et al. Identification and pyramiding of QTLs for cold tolerance at the bud bursting and the seedling stages by use of single segment substitution lines in rice (Oryza sativa L.). Mol Breeding 36, 96 (2016). https://doi.org/10.1007/s11032-016-0520-9

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  • DOI: https://doi.org/10.1007/s11032-016-0520-9

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