Skip to main content
SpringerLink
Log in

Molecular mapping of stripe rust resistance gene YrHu derived from Psathyrostachys huashanica

  • Published:
Molecular Breeding Aims and scope Submit manuscript

Abstract

Wheat stripe rust is a destructive disease that affects most wheat-growing areas worldwide. Resistance genes from related species and genera add to the genetic diversity available to wheat breeding programs. The stripe rust-resistant introgression line H9020-17-25-6-4 was developed from a cross of resistant Psathyrostachys huashanica with the susceptible wheat cultivar 7182. H9020-17-25-6-4 is resistant to all existing Chinese stripe rust races, including the three most widely virulent races, CYR32, CYR33, and V26. We attempted to characterize this new line by genomic in situ hybridization (GISH) and genetic analysis. GISH using P. huashanica genomic DNA as a probe indicated that the translocated segment was too small to be detected. Genetic analysis involving F1, F2, and F2:3 materials derived from a cross of Mingxian 169 and H9020-17-25-6-4 indicated that a single dominant gene from H9020-17-25-6-4, temporarily designated YrHu, conferred resistance to CYR29 and CYR33. A genetic map consisting of four simple sequence repeat, two sequence-tagged site (STS), and two sequence-related amplified polymorphism markers was constructed. YrHu was located on the short arm of chromosome 3A and was about 0.7 and 1.5 cM proximal to EST-STS markers BG604577 and BE489244, respectively. Both the gene and the closely linked markers could be used in marker-assisted selection.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal Biochem 196(1):80–83

    Article  CAS  PubMed  Google Scholar 

  • Cao Z, Deng Z, Wang M, Wang X, Jing J, Zhang X, Shang H, Li Z (2008) Inheritance and molecular mapping of an alien stripe-rust resistance gene from a wheat-Psathyrostachys huashanica translocation line. Plant Sci 174(5):544–549

    Article  CAS  Google Scholar 

  • Chen X (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27(3):314–337

    Article  Google Scholar 

  • Chen X (2007) Challenges and solutions for stripe rust control in the United States. Crop Pasture Sci 58:648–655

    Article  Google Scholar 

  • Chen S, Zhang A, Fu J (1991) Hybridization between Triticum and Psathyrostachys huashanica. Acta Genetica Sinica 18:508–512

    Google Scholar 

  • Chen X, Line R, Jones S (1995) Chromosomal location of genes for resistance to Puccinia striiformis in winter wheat cultivars Heines VII, Clement, Moro, Tyee, Tres, and Daws. Phytopathology 85(11):1362–1367

    Article  Google Scholar 

  • Chen W, Wu L, Liu T, Xu S, Jin S, Peng Y, Wang B (2009) Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101

    Article  Google Scholar 

  • Fu J, Wang M, Zhao J, Chen S, Hou W, Yang Q (2003) Studies on cytogenetics and utilization of wheat-Psathyrostachys huashanica medium material H8911 with resistance to wheat take-all fungus. Acta Bot Boreal Occident Sin 23:2157–2162

    Google Scholar 

  • Kosambi D (1943) The estimation of map distances from recombination values. Ann Eugen 12(1):172–175

    Article  Google Scholar 

  • Lander E, Green P, Abrahamson J, Barlow A, Daly M, Lincoln S, Newburg L (1987) MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1:174–181

    Article  CAS  PubMed  Google Scholar 

  • Li G, Quiros C (2001) Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple PCR reaction: its application to mapping and gene tagging in Brassica. Theor Appl Genet 103:455–461

    Article  CAS  Google Scholar 

  • Li Z, Zeng S (2002) Wheat rust in China. China Agriculture Press, Beijing

    Google Scholar 

  • Li Q, Chen X, Wang M, Jing J (2011) Yr45, a new wheat gene for stripe rust resistance on the long arm of chromosome 3D. Theor Appl Genet 122:189–197

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Huang J, Hou L, Liu P, Jing J, Wang B, Kang Z (2012) Genetic and molecular mapping of stripe rust resistance gene in wheat-Psathyrostachys huashanica translocation line H9020-1-6-8-3. Plant Dis 96:1482–1487

    Article  CAS  Google Scholar 

  • Lu Y, Wang M, Chen X, See D, Chao S, Jing J (2014) Mapping of Yr62 and a small-effect QTL for high-temperature adult-plant resistance to stripe rust in spring wheat PI 192252. Theor Appl Genet 127:1449–1459

    Article  CAS  PubMed  Google Scholar 

  • Ma D, Hou L, Tang M, Wang H, Li Q, Jing J (2013a) Genetic analysis and molecular mapping of a stripe rust resistance gene YrH9014 in wheat line H9014-14-4-6-1. J Integr Agric 12(4):638–645

    Article  Google Scholar 

  • Ma D, Zhou X, Hou L, Bai Y, Li Q, Wang H, Tang M, Jing J (2013b) Genetic analysis and molecular mapping of a stripe rust resistance gene derived from Psathynrostachys huashanica Keng in wheat line H9014-121-5-5-9. Mol Breed 32:365–372

    Article  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Dubcovsky J, Rogers J, Morris C, Appels R, Xia XC (2013) Catalogue of gene symbols for wheat: 2013–2014 supplement. In: 12th International wheat genetic symposium, Yokohama, Japan, 8–13 Sept 2013. http://www.shigen.nig.ac.jp/wheat/komugi/genes/download.jsp

  • Reader SM, Abbo S, Purdie KA, King IP, Miller TE (1994) Direct labelling of plant chromosomes by rapid in situ hybridization. Trends Genet 10:265–266

    Article  CAS  PubMed  Google Scholar 

  • Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114

    Article  CAS  PubMed  Google Scholar 

  • Wan A, Chen X, He Z (2007) Wheat stripe rust in China. Crop Pasture Sci 58:605–619

    Article  Google Scholar 

  • Wang M, Shang H (2000) Evaluation of resistance in Psathyrostachys huashanica to wheat take-all fungus. Acta Univ Agric Boreal Occident 28:69–71

    CAS  Google Scholar 

  • Zhao J, Ji W, Wu J, Chen X, Cheng X, Wang J, Pang Y, Liu S, Yang Q (2010) Development and identification of a wheat-Psathyrostachys huashanica addition line carrying HMW-GS, LMW-GS and gliadin genes. Genet Resour Crop Evol 57:387–394

    Article  CAS  Google Scholar 

  • Zhou X, Wang W, Wang L, Hou D, Jing J, Wang Y, Xu Z, Yao Q, Yin J, Ma D (2011) Genetics and molecular mapping of genes for high-temperature resistance to stripe rust in wheat cultivar Xiaoyan 54. Theor Appl Genet 123:431–438

    Article  CAS  PubMed  Google Scholar 

  • Zhou X, Han D, Chen X, Gou H, Guo S, Rong L, Wang Q, Huang L, Kang Z (2014a) Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34. Theor Appl Genet 127:2349–2358

    Article  CAS  PubMed  Google Scholar 

  • Zhou X, Wang M, Chen X, Lu Y, Kang Z, Jing J (2014b) Identification of Yr59 conferring high-temperature adult-plant resistance to stripe rust in wheat germplasm PI 178759. Theor Appl Genet 127:935–945

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

The research was supported by the National Basic Research Program of China (2013CB127700), National Science Foundation of China (31501620), Natural Science Funds of Hubei Province of China (2014CF367) and Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement (2014lzjj06), and the Open Project Program of State Key Laboratory of Crop Stress Biology for Arid Areas (CSBAA2015012). We thank Prof. R. A. McIntosh and Dr. Meinan Wang for critical review of the manuscript.

Author information

Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

    J. L. Yin, K. X. Chao, J. X. Jing, Q. Li & B. T. Wang

  2. Hubei Collaborative Innocvtion Center for Grain Industry, Yangtze University, Jingzhou, 434025, Hubei, People’s Republic of China

    D. F. Ma & Z. W. Fang

  3. Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement, Hubei Academy of Agriculture Science, Wuhan, 430064, Hubei, People’s Republic of China

    D. F. Ma

Authors
  1. D. F. Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Z. W. Fang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. L. Yin
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. K. X. Chao
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. X. Jing
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Q. Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. B. T. Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Z. W. Fang or B. T. Wang.

Ethics declarations

Conflicts of interest

None.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ma, D.F., Fang, Z.W., Yin, J.L. et al. Molecular mapping of stripe rust resistance gene YrHu derived from Psathyrostachys huashanica . Mol Breeding 36, 64 (2016). https://doi.org/10.1007/s11032-016-0487-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: https://doi.org/10.1007/s11032-016-0487-6

Keywords

Search

Navigation