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Quantitative trait locus analysis of unsaturated fatty acids in a recombinant inbred population of soybean

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Abstract

Soybean [Glycine max (L.) Merr.] is an important oilseed crop which produces about 30 % of the world’s edible vegetable oil. The quality of soybean oil is determined by its fatty acid composition. Soybean oil high in oleic and low in linolenic fatty acids is desirable for human consumption and other uses. The objectives of this study were to identify quantitative trait loci (QTLs) for unsaturated fatty acids and to evaluate the genetic effects of single QTL and QTL combinations in soybean. A population of recombinant inbred lines derived from the cross of SD02-4-59 × A02-381100 was evaluated for fatty acid content in seven environments. In total, 516 polymorphic single nucleotide polymorphism markers, 477 polymorphic simple sequence repeat markers and three GmFAD3 genes were used to genotype the mapping population. By using the composite interval mapping and/or the interval mapping method, a total of 15 QTLs for the three unsaturated fatty acids were detected in more than two environments. Two QTLs for oleic acid on linkage groups G [chromosome (Chr) 18] (qOLE-G) and J (Chr 16) (qOLE-J), three QTLs for linoleic acid on linkage groups A1 (Chr 5) (qLLE-A1) and G (Chr 18) (qLLE-G-1 and qLLE-G-2), and five QTLs for linolenic acid on linkage groups C2 (Chr 6), D1a (Chr 1), D1b (Chr 2), F (Chr 13) and G (Chr 18) were consistently detected in at least three individual environments and the average data over all environments. Significant QTL × QTL interactions were not detected. However, significant QTL × environment interactions were detected for all the QTLs which were repeatedly detected. Some QTLs reported previously were confirmed, and seven new QTLs (two for oleic acid, two for linoleic acid and three for linolenic acid) were identified in this study. Comparisons of two-locus and three-locus combinations indicated that cumulative effects of QTLs were significant for all the three unsaturated fatty acids. QTL pyramiding by molecular marker-assisted breeding would be an appropriate strategy for the improvement of unsaturated fatty acids in soybean.

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Acknowledgments

This research was supported in part by USB Seed Composition Research project via USDA/ARS, and South Dakota Soybean Research and Promotion Council project. The determination of fatty acids was completed in the USDA/ARS Lab., in Peoria, IL and the Iowa State University DNA Facility in Ames, IA. Genotyping of three GmFAD3 genes was performed by Dr. Kristin Bilyeu’s Lab., USDA/ARS in Columbia, MO. The parental line A02-381100 was provided by Dr. Walter Fehr, Iowa State University. Dr. Kelly Whiting, the United Soybean Board, reviewed the manuscript and provided constructive suggestions.

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  1. David L. Hyten

    Present address: Pioneer Hi-Bred International, 7300 NW 62nd Ave., PO Box 1004, Johnston, IA, 50131, USA

Authors and Affiliations

  1. Plant Science Department, South Dakota State University, Northern Plains Biostress Laboratory Building, Box 2140C, Brookings, SD, 57007, USA

    Xianzhi Wang, Guo-Liang Jiang & Marci Green

  2. Crop Production and Protection, GWCC-BLTSVL, USDA/ARS, 5601 Sunnyside Ave., Beltsville, MD, 20705-5139, USA

    Roy A. Scott

  3. Soybean Genomics and Improvement Laboratory, USDA/ARS, 10300 Baltimore Ave., Beltsville, MD, 20705-2350, USA

    David L. Hyten & Perry B. Cregan

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  1. Xianzhi Wang
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Correspondence to Guo-Liang Jiang.

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Wang, X., Jiang, GL., Green, M. et al. Quantitative trait locus analysis of unsaturated fatty acids in a recombinant inbred population of soybean. Mol Breeding 33, 281–296 (2014). https://doi.org/10.1007/s11032-013-9948-3

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