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QTL associated with horizontal resistance to soybean cyst nematode in Glycine soja PI464925B

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Abstract

Soybean cyst nematode (Heterodera glycines Ichinohe; SCN) is the primary disease responsible for yield loss of soybean [Glycine max (L.) Merr.]. Resistant cultivars are an effective management tool; however, the sources currently available have common resistant genes. Glycine soja Sieb. and Zucc., the wild ancestor of domesticated soybean, represents a diverse germplasm pool with known SCN resistance. The objectives of this research were to: (1) determine the genetic variation and inheritance of SCN resistance in a G. max (‘S08-80’) × G. soja (PI464925B) F 4:5 recombinant inbred line (RIL) population; and (2) identify and evaluate quantitative trait loci (QTL) associated with SCN resistance. Transgressive segregation for resistance was observed, although neither parent was resistant to the Chatham and Ruthven SCN isolates. Broad sense heritability was 0.81 for the Ruthven and 0.91 for the Chatham isolate. Root dry weight was a significant covariate that influenced cyst counts. One RIL [female index (FI) = 5.2 ± 1.11] was identified as resistant to the Chatham isolate (FI < 10). Seventeen and three RILs infected with Chatham and Ruthven isolates, respectively, had mean adjusted cyst counts of zero. Unique and novel QTL, which derived resistance from G. soja, were identified on linkage groups I, K, and O, and individually explained 8, 7 and 5% (LOD = 2.1–2.7) of the total phenotypic variation, respectively. Significant epistatic interactions were found between pairs of SSR markers that individually may or may not have been associated with SCN resistance, which explained between 10 and 15% of the total phenotypic variation. Best-fit regression models explained 21 and 31% of the total phenotypic variation in the RIL population to the Chatham and Ruthven isolates, respectively. The results of this study help to improve the understanding of the genetic control of SCN resistance in soybean caused by minor genes resulting in horizontal resistance. The incorporation of the novel resistance QTL from G. soja could increase the durability of SCN-resistance in soybean cultivars, especially if major gene resistance breaks down.

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Acknowledgments

The authors would like to thank Dr. Gordon Hoover and Mr. Chris Grainger for technical assistance, Drs. Irena Rajcan, Elizabeth Lee, and Brian Allen for assistance with statistical analysis of the data, and ACCESS Plant Technology, Inc. for CystX seed. This research was funded by the University of Guelph Food Systems Biotechnology Centre, Ontario Soybean Growers, and the Ontario Ministry of Agriculture Food and Rural Affairs.

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Authors and Affiliations

  1. Department of Plant Agriculture, University of Guelph, N1G 2W1, Guelph, ON, Canada

    Shawn M. J. Winter, Barry J. Shelp & Istvan Rajcan

  2. Greenhouse and Processing Crop Research Center, Agriculture and Agri-Food Canada, N0R 1G0, Harrow, ON, Canada

    Terry R. Anderson & Tom W. Welacky

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  1. Shawn M. J. Winter
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  2. Barry J. Shelp
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Correspondence to Istvan Rajcan.

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Communicated by C. Gebhardt.

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Winter, S.M.J., Shelp, B.J., Anderson, T.R. et al. QTL associated with horizontal resistance to soybean cyst nematode in Glycine soja PI464925B. Theor Appl Genet 114, 461–472 (2007). https://doi.org/10.1007/s00122-006-0446-4

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