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Pyramided QTL underlying tolerance to Phytophthora root rot in mega-environments from soybean cultivars ‘Conrad’ and ‘Hefeng 25’

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Abstract

Phytophthora root rot (PRR) of soybean (Glycine max (L.) Merr.) is the second most important cause of yield loss by disease in North America, surpassed only by soybean cyst nematode (Wrather et al. in Can J Plant Pathol 23:115–121, 2001). Tolerance can provide economically useful disease control, conditioning partial resistance of soybean to PRR. The aims of this study were to identify new quantitative trait loci (QTL) underlying tolerance to PRR, and to evaluate the effects of pyramided or stacked loci on the level of tolerance. A North American cultivar ‘Conrad’ (tolerant to PRR) was crossed with a northeastern China cultivar ‘Hefeng 25’ (tolerant to PRR). Through single-seed descent, 140 F2:5 and F2:6 recombinant inbred lines were advanced. A total of 164 simple sequence repeat (SSR) markers were used to construct a genetic linkage map. The percentage of seedling death was measured over 2 years (2007 and 2008) in the field at four naturally infested locations in Canada and China following additional soil infestation and in the greenhouse following inoculation with Phytophthora sojae isolate. A total of eight QTL underlying tolerance to PRR were identified, located in five linkage groups (F, D1b+w, A2, B1, and C2). The phenotypic variation contributed by the loci ranged from 4.24 to 27.98%. QPRR-1 (anchored in the interval of SSR markers Satt325 and Satt343 of LG F), QPRR-2 (anchored in the interval of Satt005 and Satt600 of LG D1b+w), and QPRR-3 (anchored in the interval of Satt579 and Sat_089 of LG D1b+w) derived their beneficial allele from ‘Conrad’. They were located at chromosomal locations known to underlie PRR tolerance in diverse germplasm. Five QTL that derived beneficial alleles from ‘Hefeng 25’ were identified. The QTL (QPRR-1 to QPRR-7) that were detected across at least three environments were selected for loci stacking and to analyze the relationship between number of tolerance loci and disease loss percentage. The accumulation of tolerance loci was positively correlated with decreases in disease loss percentage. The pyramid of loci underlying tolerance to PRR provided germplasm useful for crop improvement by marker-assisted selection and may provide durable cultivar tolerance against the PRR disease.

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Acknowledgments

This study was conducted in the Key Laboratory of Soybean Biology of Chinese Education Ministry and Soybean Development Centre of Agricultural Ministry, financially supported by National High Technology Project (2006AA10Z1F1 and 2006AA100104-4), 948 project of Agricultural Ministry of China (2006-G5), National Nature Science Foundation projects (30971810, 60932008), National 973 Project (2009CB118400), Technology project of Education Ministry of Heilongjiang Province (11541025).

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Authors and Affiliations

  1. Soybean Research Institute, Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, 150030, Harbin, China

    Xiuping Li, Yingpeng Han, Weili Teng, Shuzheng Zhang & Wenbin Li

  2. Greenhouse and Processing Crops Research Centre, Agriculture and Agri-Food Canada, Harrow, ON, N0R 1G0, Canada

    Kangfu Yu, Vaino Poysa & Terry Anderson

  3. College of Life Sciences, Jiamusi University, 154007, Jiamusi, China

    Xiuping Li

  4. Agricultural Academy of Heilongjiang Province, 154008, Jiamusi, China

    Junjie Ding

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  1. Xiuping Li
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  2. Yingpeng Han
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  3. Weili Teng
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  4. Shuzheng Zhang
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  9. Wenbin Li
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Correspondence to Wenbin Li.

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Communicated by D. Lightfoot.

X. Li and Y. Han have equal contributions to the paper.

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Li, X., Han, Y., Teng, W. et al. Pyramided QTL underlying tolerance to Phytophthora root rot in mega-environments from soybean cultivars ‘Conrad’ and ‘Hefeng 25’. Theor Appl Genet 121, 651–658 (2010). https://doi.org/10.1007/s00122-010-1337-2

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