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Co-cultivation and stepwise cultivation of Chaetoceros muelleri and Amphora sp. for fucoxanthin production under gradual salinity increase

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Abstract

In a seawater-based open pond microalgae cultivation system salinity will increase gradually over time due to evaporative loss. Continuous salinity increase would lead to non-optimal salinities which negatively affect the biomass and fucoxanthin productivity. To increase and maintain high overall biomass and fucoxanthin productivity, even in the non-optimal salinity zone, two cultivation methods for marine and halotolerant microalgae were carried out, co-cultivation and stepwise cultivation (sequential cultivation). Two fucoxanthin-producing diatoms, Chaetoceros muelleri (marine) and Amphora sp. (halotolerant), were cultivated at non-optimal salinities between 59 and 65‰. Stepwise cultivation showed approximately 63% higher total biomass and 47% higher fucoxanthin productivity than that of co-culture. The ability to reutilize culture media in the stepwise cultivation increases the sustainability of that method. The use of a stepwise culture regime, coupled with a regimen of gradually increasing salinity, provides the possibility of year round fucoxanthin production from microalgae.

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Acknowledgements

We are thankful to Emeka G. Nwoba, PhD student, Algae R& D Centre, School of Veterinary and Life Sciences, Murdoch University, Western Australia, for his assistance in extracting fucoxanthin and lipid.

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Authors and Affiliations

  1. Algae R&D Centre, School of Veterinary and Life Sciences, Murdoch University, Murdoch, Western Australia, 6150, Australia

    Tasneema Ishika & Navid R Moheimani

  2. School of Engineering and Information Technology, Murdoch University, Murdoch, Western Australia, 6150, Australia

    Damian W Laird & Parisa A Bahri

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  1. Tasneema Ishika
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  2. Damian W Laird
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  3. Parisa A Bahri
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  4. Navid R Moheimani
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Correspondence to Navid R Moheimani.

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Ishika, T., Laird, D.W., Bahri, P.A. et al. Co-cultivation and stepwise cultivation of Chaetoceros muelleri and Amphora sp. for fucoxanthin production under gradual salinity increase. J Appl Phycol 31, 1535–1544 (2019). https://doi.org/10.1007/s10811-018-1718-5

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  • DOI: https://doi.org/10.1007/s10811-018-1718-5

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