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Improvement of biomass and fatty acid productivity in ocean cultivation of Tetraselmis sp. using hypersaline medium

  • 6th Congress of the International Society for Applied Phycology
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Abstract

In this study, hypersaline media were used for ocean cultivation of the marine microalga Tetraselmis sp. KCTC12432BP for enhanced biomass and fatty acid (FA) productivity. Hypersaline media (55, 80, and 105 PSU) were prepared without sterilization by addition of NaCl to seawater obtained from Incheon, Korea. The highest biomass productivity was obtained at 55 PSU (0.16 g L−1 day−1) followed by 80 PSU (0.15 g L−1 day−1). Although the specific growth rate of Tetraselmis decreased at salinities higher than 55 PSU, prevention of contamination led to higher biomass productivity at 80 PSU than at 30 PSU (0.03 g L−1 day−1). FA content of algal biomass increased as salinity increased to 80 PSU, above which it declined, and FA productivity was highest at 80 PSU. Ocean cultivation of Tetraselmis was performed using 50-L tubular module photobioreactors and 2.5-kL square basic ponds, closed- and open-type ocean culture systems, respectively. Culturing microalgae in hypersaline medium (80 PSU) improved biomass productivities by 89 and 152% in closed and open cultures, respectively, compared with cultures with regular salinity. FA productivity was greatly improved by 369% in the closed cultures. The efficacy of salinity shift and N-deficiency to enhance FA productivity was also investigated. Lowering salinity to 30 PSU with N-starvation following cultivation at 80 PSU improved FA productivity by 19% in comparison with single-stage culture without N-deficiency at 30 PSU. The results show that salinity manipulation could be an effective strategy to improve biomass and FA productivity in ocean cultivation of Tetraselmis sp.

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References

  • Al-Hasan RH, Ali AM, Ka'wash HH, Radwan SS (1990) Effect of salinity on the lipid and fatty acid composition of the halophyte Navicula sp.: potential in mariculture. J Appl Phycol 2:215–222

    Article  Google Scholar 

  • Bartley ML, Boeing WJ, Corcoran AA, Holguin FO, Schaub T (2013) Effects of salinity on growth and lipid accumulation of biofuel microalga Nannochloropsis salina and invading organisms. Biomass Bioenergy 54:83–88

    Article  CAS  Google Scholar 

  • Borowitzka MA (1999) Commercial production of microalgae: ponds, tanks, tubes and fermenters. J Biotech 70:313–321

    Article  CAS  Google Scholar 

  • Carney LT, Reinsch SS, Lane PD, Solberg OD, Jansen LS, Williams KP, Trent JD, Lane TW (2014) Microbiome analysis of a microalgal mass culture growing in municipal wastewater in a prototype OMEGA photobioreactor. Algal Res 4:52–61

    Article  Google Scholar 

  • Das P, Aziz SS, Obbard JP (2011) Two phase microalgae growth in the open system for enhanced lipid productivity. Renew Energy 36:2524–2528

    Article  CAS  Google Scholar 

  • Fanesi A, Raven JA, Giordano M (2014) Growth rate affects the responses of the green alga Tetraselmis suecica to external perturbations. Plant Cell Environ 37:512–519

    Article  CAS  Google Scholar 

  • Fon-Sing S, Borowitzka MA (2016) Isolation and screening of euryhaline Tetraselmis spp. suitable for large-scale outdoor culture in hypersaline media for biofuels. J Appl Phycol 28:1–14

    Article  CAS  Google Scholar 

  • Fon-Sing S, Isdepsky A, Borowitzka MA, Lewis D (2014) Pilot-scale continuous recycling of growth medium for the mass culture of a halotolerant Tetraselmis sp. in raceway ponds under increasing salinity: a novel protocol for commercial microalgal biomass production. Bioresour Technol 161:47–54

    Article  CAS  Google Scholar 

  • Gleason FH, Karpov SA, Lilje O, Macarthur DJ, Van Otgen F, Sime-Ngando T (2014) Zoosporic parasites of phytoplankton. In: Gareth Jones EB, Hyde KD, Pang K-L (eds) Freshwater fungi and fungal-like organisms, Walter de Gruyter​, Berlin, pp 279-304

  • Gu N, Lin Q, Li G, Tan Y, Huang L, Lin J (2012) Effect of salinity on growth, biochemical composition, and lipid productivity of Nannochloropsis oculata CS 179. Eng Life Sci 12:631–637

    Article  CAS  Google Scholar 

  • Hagemann M (2016) Coping with high and variable salinity: molecular aspects of compatible solute accumulation. In: Borowitzka MA, Beardall J, Raven JA (eds) The physiology of microalgae. Springer, Cham, pp 359-372.

    Chapter  Google Scholar 

  • Hellebust JA (1985) Mechanisms of response to salinity in halotolerant microalgae. Plant Soil 89:69–81

    Article  Google Scholar 

  • Ho S-H, Nakanishi A, Ye X, Chang J-S, Hara K, Hasunuma T, Kondo A (2014) Optimizing biodiesel production in marine Chlamydomonas sp. JSC4 through metabolic profiling and an innovative salinity-gradient strategy. Biotechnol Biofuels 7:97

    Article  Google Scholar 

  • Hoffman Y, Aflalo C, Zarka A, Gutman J, James TY, Boussiba S (2008) Isolation and characterization of a novel chytrid species (phylum Blastocladiomycota), parasitic on the green alga Haematococcus. Mycol Res 112:70–81

    Article  CAS  Google Scholar 

  • Karpov SA, Mamkaeva MA, Benzerara K, Moreira D, López-García P (2014) Molecular phylogeny and ultrastructure of Aphelidium aff. melosirae (Aphelida, Opisthosporidia). Protist 165:512–526

    Article  Google Scholar 

  • Khatoon H, Rahman NA, Banerjee S, Harun N, Suleiman SS, Zakaria NH, Lananan F, Hamid SHA, Endut A (2014) Effects of different salinities and pH on the growth and proximate composition of Nannochloropsis sp. and Tetraselmis sp. isolated from South China Sea cultured under control and natural condition. Int Biodeterior Biodegrad 95:11–18

    Article  CAS  Google Scholar 

  • Kim G, Lee C-H, Lee K (2016a) Enhancement of lipid production in marine microalga Tetraselmis sp. through salinity variation. Korean J Chem Eng 33:230–237

    Article  CAS  Google Scholar 

  • Kim Z-H, Park H, Hong S-J, Lim S-M, Lee C-G (2016b) Development of a floating photobioreactor with internal partitions for efficient utilization of ocean wave into improved mass transfer and algal culture mixing. Bioprocess Biosyst Eng 39:713–723

    Article  CAS  Google Scholar 

  • Kim Z-H, Park H, Lee C-G (2016c) Seasonal assessment of biomass and fatty acid productivity by Tetraselmis sp. in the ocean using semi-permeable membrane photobioreactors. J Microbiol Biotechnol 26:1098–1102

    Article  CAS  Google Scholar 

  • Kim Z-H, Park H, Lee H-S, Lee C-G (2016d) Enhancing photon utilization efficiency for astaxanthin production from Haematococcus lacustris using a split-column photobioreactor. J Microbiol Biotechnol 26:1285–1289

    Article  CAS  Google Scholar 

  • Kim Z-H, Park H, Ryu Y-J, Shin D-W, Hong S-J, Tran H-L, Lim S-M, Lee C-G (2015) Algal biomass and biodiesel production by utilizing the nutrients dissolved in seawater using semi-permeable membrane photobioreactors. J Appl Phycol 27:1763–1773

    Article  CAS  Google Scholar 

  • Klok AJ, Lamers PP, Martens DE, Draaisma RB, Wijffels RH (2014) Edible oils from microalgae: insights in TAG accumulation. Trends Biotechnol 32:521–528

    Article  CAS  Google Scholar 

  • Moheimani NR (2013) Inorganic carbon and pH effect on growth and lipid productivity of Tetraselmis suecica and Chlorella sp (Chlorophyta) grown outdoors in bag photobioreactors. J Appl Phycol 25:387–398

    Article  CAS  Google Scholar 

  • Norsker N-H, Barbosa MJ, Vermuë MH, Wijffels RH (2011) Microalgal production—a close look at the economics. Biotechnol Adv 29:24–27

    Article  CAS  Google Scholar 

  • Novoveská L, Zapata AKM, Zabolotney JB, Atwood MC, Sundstrom ER (2016) Optimizing microalgae cultivation and wastewater treatment in large-scale offshore photobioreactors. Algal Res 18:86–94

    Article  Google Scholar 

  • Park H, Lee C-G (2016) Theoretical calculations on the feasibility of microalgal biofuels: utilization of marine resources could help realizing the potential of microalgae. Biotechnol J 11:1461–1470

    Article  CAS  Google Scholar 

  • Pretorius J, Engelbrecht AHP (1980) The occurrence of a fungal parasite on a Tetraselmis (Prasinophyceae) species. Water SA 6:181–185

    Google Scholar 

  • Renaud SM, Parry DL (1994) Microalgae for use in tropical aquaculture II: effect of salinity on growth, gross chemical composition and fatty acid composition of three species of marine microalgae. J Appl Phycol 6:347–356

    Article  CAS  Google Scholar 

  • Richardson JW, Johnson MD, Zhang X, Zemke P, Chen W, Hu Q (2014) A financial assessment of two alternative cultivation systems and their contributions to algae biofuel economic viability. Algal Res 4:96–104

    Article  Google Scholar 

  • Rodolfi L, Chini Zittelli G, Bassi N, Padovani G, Biondi N, Bonini G, Tredici MR (2009) Microalgae for oil: strain selection, induction of lipid synthesis and outdoor mass cultivation in a low-cost photobioreactor. Biotechnol Bioeng 102:100–112

    Article  CAS  Google Scholar 

  • Saadaoui I, Al Ghazal G, Bounnit T, Al Khulaifi F, Al Jabri H, Potts M (2016) Evidence of thermo and halotolerant Nannochloris isolate suitable for biodiesel production in Qatar Culture Collection of Cyanobacteria and Microalgae. Algal Res 14:39–47

    Article  Google Scholar 

  • Su C-H, Chien L-J, Gomes J, Lin Y-S, Yu Y-K, Liou J-S, Syu R-J (2011) Factors affecting lipid accumulation by Nannochloropsis oculata in a two-stage cultivation process. J Appl Phycol 23:903–908

    Article  CAS  Google Scholar 

  • Suh IS, Joo H-N, Lee C-G (2006) A novel double-layered photobioreactor for simultaneous Haematococcus pluvialis cell growth and astaxanthin accumulation. J Biotech 125:540–546

    Article  CAS  Google Scholar 

  • Takagi M, Karseno YT (2006) Effect of salt concentration on intracellular accumulation of lipids and triacylglyceride in marine microalgae Dunaliella cells. J Biosci Bioeng 101:223–226

    Article  CAS  Google Scholar 

  • Tran H-L, Hong S-J, Lee C-G (2009) Evaluation of extraction methods for recovery of fatty acids from Botryococcus braunii LB 572 and Synechocystis sp. PCC 6803. Biotechnol Bioprocess Eng 14:187–192

    Article  CAS  Google Scholar 

  • Wang D, Wang W, Xu N, Sun X (2016) Changes in growth, carbon and nitrogen enzyme activity and mRNA accumulation in the halophilic microalga Dunaliella viridis in response to NaCl stress. J Ocean Univ China 15:1094–1100

    Article  CAS  Google Scholar 

  • Wiley P, Harris L, Reinsch S, Tozzi S, Embaye T, Clark K, McKuin B, Kolber Z, Adams R, Kagawa H (2013) Microalgae cultivation using offshore membrane enclosures for growing algae (OMEGA). J Sust Bioenergy Syst 3:18–32

    Article  Google Scholar 

  • Xia L, Ge H, Zhou X, Zhang D, Hu C (2013) Photoautotrophic outdoor two-stage cultivation for oleaginous microalgae Scenedesmus obtusus XJ-15. Bioresour Technol 144:261–267

    Article  CAS  Google Scholar 

  • Zakhozhii I, Matalin D, Popova L, Balnokin YV (2012) Responses of photosynthetic apparatus of the halotolerant microalga Dunalliella maritima to hyperosmotic salt shock. Russ J Plant Physiol 59:42–49

    Article  CAS  Google Scholar 

  • Zeng M-T, Vonshak A (1998) Adaptation of Spirulina platensis to salinity-stress. Comp Biochem Physiol A 120:113–118

    Article  Google Scholar 

Download references

Funding

This research was supported by a grant from the Marine Biotechnology Program (Project No.: 20090267, Development of Marine Microalgal Biofuel Production Technology) funded by the Ministry of Oceans and Fisheries, Korea. Authors are also grateful to the Manpower Development Program for Marine Energy from the same ministry (Project No.: 201405502). Hanwool Park was also supported by a NRF (National Research Foundation of Korea) Grant funded by the Korean Government (NRF-2013H1A2A1032623).

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Author notes

  1. Hanwool Park and Daewoo Jung contributed equally to this work.

Authors and Affiliations

  1. Department of Marine Science & Biological Engineering, Inha University, Incheon, 22212, South Korea

    Hanwool Park, Daewoo Jung, Jongchan Lee, Philhan Kim, Injae Jung, Sang-Min Lim & Choul-Gyun Lee

  2. National Marine Bioenergy R&D Center, Inha University, Incheon, 22212, South Korea

    Hanwool Park, Daewoo Jung, Jongchan Lee, Philhan Kim, Yonghee Cho, Injae Jung, Sang-Min Lim & Choul-Gyun Lee

  3. Culture Techniques Division, Freshwater Bioresources Culture Research Division, Nakdonggang National Institute of Biological Resources, Sangju, 37242, South Korea

    Z-Hun Kim

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  1. Hanwool Park
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Correspondence to Choul-Gyun Lee.

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Park, H., Jung, D., Lee, J. et al. Improvement of biomass and fatty acid productivity in ocean cultivation of Tetraselmis sp. using hypersaline medium. J Appl Phycol 30, 2725–2735 (2018). https://doi.org/10.1007/s10811-018-1388-3

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  • DOI: https://doi.org/10.1007/s10811-018-1388-3

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