Abstract
Oligosaccharides can elicit a defense-related response in marine algae or seaweeds. This study aimed to investigate the possible application of oligosaccharides as biological elicitors for algal farming by measuring their antirotting effects on Pyropia haitanensis. The concentration of oligoagars and the time of treatment were optimized by examining their effects on the percentage of rotting in thalli. Oxidative burst and expression of defense-related genes were examined. The optimized conditions obtained were then applied in an aquaculture setting for P. haitanensis. Elicitation with oligoagars at 100 μg mL−1 for 2 h produced the optimal rot resistance effect, and oligoagars delayed the severe rotting of thalli by more than 13 days. Oligoagars elicited rapid respiration in thalli. The expression levels of Phrboh and Phhsp70 genes were up-regulated whereas Phsod was down-regulated. During aquaculture, treatment with oligoagars when thalli were 30 days old and when conchospores adhered to nets improved growth, increased yield of products, and delayed the maturation of thalli. However, treatment when conchospores adhered to nets had better results and was more convenient and practical. Overall, we found that oligoagars have potential applications as elicitors for biological farming and can be applied in the aquaculture of P. haitanensis with promising results.
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Acknowledgements
This study was supported by the National Key Technology Research and Development Program of the Ministry of Science and Technology of China (No. 2011BAD13B08), the Science and Technology Major Project of the Ministry of Science and Technology of Zhejiang, China (No. 2012C12907-6), the Zhejiang Open Foundation of the Most Important Subjects (xkzsc1419), the Ningbo Programs for Science and Technology Development (2014C10044), the K.C. Wong Magna Fund of Ningbo University, the Zhejiang 151 Talents Project, and the Open Fund of the Zhejiang Provincial Top Key Discipline of Aquaculture at Ningbo University. We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.
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Chen, H., Jian, Q., Luo, Q. et al. Application of oligoagars as elicitors for field aquaculture of Pyropia haitanensis . J Appl Phycol 28, 1783–1791 (2016). https://doi.org/10.1007/s10811-015-0734-y
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DOI: https://doi.org/10.1007/s10811-015-0734-y