Abstract
Heat shock protein 70s (Hsp70s) play particularly important roles in a variety of stress responses and have been widely studied in many higher plants and algae. Pyropia haitanensis blades inhabit the upper intertidal zones of rocky shores and are exposed to dynamic environmental changes associated with the turning tides, including desiccation and high-temperature stress. In this study, based on unigene sequences of P. haitanensis, five full-length PhHsp70 genes were obtained by rapid amplification of complementary DNA (cDNA) ends or direct PCR and named PhHsp70-1, PhHsp70-2, PhHsp70-3, PhHsp70-4, and PhHsp70-5. The full-length cDNA of the five PhHsp70 genes comprised 2,249, 2,380, 1,945, 2,177, and 2,327 nucleotides, respectively. The cDNAs encoded proteins of 664, 668, 621, 654, and 686 amino acids, with isoelectric points of 4.93, 5.17, 4.87, 5.09, and 5.04, respectively. On the basis of conserved motifs and phylogenetic tree analysis, the five PhHsp70s could be divided into four subfamilies, two in the cytoplasmic subfamily and one for each in the chloroplast, mitochondria, and endoplasmic reticulum subfamilies. The expressions of the five PhHsp70 genes, as measured by real-time quantitative PCR, were significantly induced by high-temperature stress but had different expression patterns under desiccation stress. These results suggested that PhHsp70s play important roles in the response to high-temperature stress but have no obvious functions in adaptation to desiccation stress, except under extreme desiccation conditions.
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Acknowledgments
This research was supported in part by the 863 Project of China (grant no. 2012AA10A411), the National Natural Science Foundation of China (grant nos. 41176151 and 41276177), and the National Marine Public Welfare Research Project (grant nos. 201105008 and 201105023).
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Ji, D., Li, B., Xu, Y. et al. Cloning and quantitative analysis of five heat shock protein 70 genes from Pyropia haitanensis . J Appl Phycol 27, 499–509 (2015). https://doi.org/10.1007/s10811-014-0314-6
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DOI: https://doi.org/10.1007/s10811-014-0314-6