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NADPH oxidases in Eukaryotes: red algae provide new hints!

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Abstract

The red macro-alga Chondrus crispus is known to produce superoxide radicals in response to cell-free extracts of its green algal pathogenic endophyte Acrochaete operculata. So far, no enzymes involved in this metabolism have been isolated from red algae. We report here the isolation of a gene encoding a homologue of the respiratory burst oxidase gp91phox in C. crispus, named Ccrboh. This single copy gene encodes a polypeptide of 825 amino acids. Search performed in available genome and EST algal databases identified sequences showing common features of NADPH oxidases in other algae such as the red unicellular Cyanidioschyzon merolae, the economically valuable red macro-alga Porphyra yezoensis and the two diatoms Phaeodactylum tricornutum and Thalassiosira pseudonana. Domain organization and phylogenetic relationships with plant, animal, fungal and algal NADPH oxidase homologues were analyzed. Transcription analysis of the C. crispus gene revealed that it was over-transcribed during infection of C. crispus gametophyte by the endophyte A. operculata, and after incubation in presence of atrazine, methyl jasmonate and hydroxyperoxides derived from C20 polyunsaturated fatty acids (PUFAs). These results also illustrate the interest of exploring the red algal lineage for gaining insight into the deep evolution of NADPH oxidases in Eukaryotes.

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Acknowledgements

We thank N. Kusuhara for generously providing the clone AV429771 from the P. yezoensis EST database, C. Bowler for giving us access to the diatoms sequences and K. Jabbari for having carried out the diatom databases searches. We also thank E. Billard and D. Scornet for technical support and B. Charrier for her helpful advice on real-time PCR experiments. We are grateful to anonymous reviewers whose sharp comments greatly helped us in improving the final manuscript C. Hervé was supported by a BDI fellowship from the Centre National de la Recherche Scientifique. Part of this work was performed in the frame of “Marine Genomics Europe” NoE (EC contract No GOCE-CT-2004-505403).

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  1. UMR7139 (CNRS-UPMC-LIA DIAMS), Station Biologique, F-29682, Roscoff cedex, France

    Cécile Hervé, Thierry Tonon, Jonas Collén, Erwan Corre & Catherine Boyen

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  1. Cécile Hervé
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  2. Thierry Tonon
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Correspondence to Catherine Boyen.

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Communicated by F.-A. Wollman

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Hervé, C., Tonon, T., Collén, J. et al. NADPH oxidases in Eukaryotes: red algae provide new hints!. Curr Genet 49, 190–204 (2006). https://doi.org/10.1007/s00294-005-0044-z

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