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Time-course of ionic responses and proteomic analysis of a Tibetan wild barley at early stage under salt stress

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Abstract

Barley (Hordeum vulgare L.) is well known for its relatively high salt tolerance among cereal crops. However, the genetic variation of cultivated barley becomes narrower due to continuous artificial selection and breeding processes. Compared with cultivated barley, wild barley contains wider genetic variation and abundant sources for abiotic stress tolerance, considering as an elite resource for mechanism study on salt tolerance. In this study, Tibetan wild barley accession XZ113 identified with high salt tolerance, was used to investigate ionic responses and to identify proteins involved in salt tolerance in roots and shoots at early stage of salt stress, during 48 h. Exposed to salinity, shoot growth is more sensitive than root growth. Conversely, K/Na ratio in the shoots was larger than that in the roots, and both were above 1.0. Steady-state K+ flux experiment showed XZ113 had a strong K+-retaining ability under salt stress, maybe contributing to its good performance of the absolute growth rate. Proteomic results suggested that monodehydroascorbate reductase and peroxidases related to reactive oxygen species scavenging in the roots and phosphoglycerate kinase, triosephosphate isomerase and sedoheptulose-1,7-bisphosphatase associated with photosynthesis and metabolisms in the shoots, played important roles in salt tolerance at early stage of salinity in wild barley.

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Abbreviations

ATP:

Adenosine triphosphate

BPB:

Bromophenol blue

DTT:

Dithiothreitol

DW:

Dried weight

FW:

Fresh weight

GR:

Growth rate

ICP–OES:

Inductively coupled plasma–optical emission spectrometer

IEF:

Isoelectric focusing electrophoresis

IPG:

Immobilized pH gradient

MALDI-TOF/TOF MS:

Matrix-assisted laser desorption/ionization time of flight mass

MIFE:

Microelectrode ion flux estimation

PM:

Plasma membrane

PIPs:

Plasma membrane intrinsic proteins

ROS:

Reactive oxygen species

RWC:

Relative water content

SDS-PAGE:

Sodium dodecyl sulfate polyacrylamide gel electrophoresis

SBPase:

Sedoheptulose-1,7-bisphosphatase

2-DE:

Two-dimensional gel electrophoresis

TCA:

Tricarboxylic acid cycle

VDAC:

Voltage-dependent anion channel

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Acknowledgments

We are grateful to Prof. Dongfa Sun (Huazhong Agricultral University, China) for providing seeds of Tibetan wild barley accession. This research was supported by Natural Science Foundation of China (31330055, 31301246), China Agriculture Research System (CARS-05) and the Fundamental Research Funds for the Central Universities (2016QNA6013).

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Authors and Affiliations

  1. Department of Agronomy, Key Laboratory of Crop Germplasm Resource of Zhejiang Province, Zhejiang University, Hangzhou, 310058, China

    Qiufang Shen, Liangbo Fu, Feng Xue, Guoping Zhang & Dezhi Wu

  2. Life Science and Technology Center, China Seed Group Co., Ltd., Wuhan, 430070, China

    Long Qiu

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  1. Qiufang Shen
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  2. Liangbo Fu
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  3. Long Qiu
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  4. Feng Xue
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  5. Guoping Zhang
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  6. Dezhi Wu
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Correspondence to Dezhi Wu.

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Shen, Q., Fu, L., Qiu, L. et al. Time-course of ionic responses and proteomic analysis of a Tibetan wild barley at early stage under salt stress. Plant Growth Regul 81, 11–21 (2017). https://doi.org/10.1007/s10725-016-0180-0

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  • DOI: https://doi.org/10.1007/s10725-016-0180-0

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