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Ionic relations and proline accumulation in shoots of two Chinese Iris germplasms during NaCl stress and subsequent relief

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Abstract

Adaptation to salinity in plant includes not only their response to stress, but also their ability to recover from it. Seeds of Chinese Iris (Iris lactea var. chinensis) collected randomly from an arid area in inland province of Xinjiang (Xj, E93°30′, N42°48′, 740 m), and a semi-arid coastal area in Beijing Municipality (Bj, N40°34′, E116°10′, 540 m) were studied. The objectives were to determine differences between germplasms in ionic relations or accumulation of proline in the shoots and establish how I. lactea adapts to salt stress and subsequent relief. Hydroponically-grown seedlings of the two germplasms were supplied with nutrient solutions containing 0.1 (control), 140 and 280 mM NaCl for 12 days, and supplied subsequently with a non-saline control solution for 12 days to relieve NaCl stress. Germplasm Xj had larger shoot mass and shoot/root ratio than Bj during stress and recovery periods. Salinity, even subsequent recovery, more adversely affected the water content in Bj than Xj. Under identical provision of treatments, the Xj maintained relatively higher K+/Na+ ratio and selectivity for transportation of K+ over Na+ than Bj. Additionally, we observed a slight proline accumulation in shoots of Bj, but its content was insufficient for lowering the solute potential. Germplasm Xj exhibits better adaptation to salinity and subsequent recovery. This is mainly due to its higher selectivity for transporting K+ over Na+. Proline accumulation did not play a key role the osmotic adjustment of the two I. lactea germplasms studied.

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Acknowledgments

This work was supported financially by the National Key Basic Research Program of China (2009CB825101) and the National Natural Science Foundation of China (30870237). We are thankful to Professor Anthony Egrinya Eneji (University of Calabar, Nigeria) and Hai-Min Wang (College of Humanities and Development, China Agricultural University, China) for their critical reading and revision of the manuscript.

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Authors and Affiliations

  1. Key Laboratory of Arable Land Conservation (North China), Ministry of Agriculture, College of Resources and Environmental Sciences, China Agricultural University, Beijing, 100193, China

    Biao Zhang & Pin-Fang Li

  2. Beijing Oasis Technology Development Co., Ltd, Beijing, 100193, China

    Fen-Cheng Fan

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  1. Biao Zhang
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  2. Pin-Fang Li
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  3. Fen-Cheng Fan
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Correspondence to Pin-Fang Li.

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Zhang, B., Li, PF. & Fan, FC. Ionic relations and proline accumulation in shoots of two Chinese Iris germplasms during NaCl stress and subsequent relief. Plant Growth Regul 68, 49–56 (2012). https://doi.org/10.1007/s10725-012-9692-4

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  • DOI: https://doi.org/10.1007/s10725-012-9692-4

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