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Nitric oxide can induce tolerance to oxidative stress of peanut seedlings under cadmium toxicity

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Abstract

To study the role of sodium nitroprusside (SNP, a donor of NO) in alleviating cadmium (Cd) toxicity in peanut (Arachis hypogaea L.), peanut seedlings exposed to 50, 100, or 200 µM Cd as CdCl2 were treated with 250 µM SNP. Cd exposure depressed plant growth, inhibited the photosynthesis, and resulted in oxidative stress. In roots, Cd was mostly trapped in the cell wall under low Cd stress, but most Cd was accumulated in the soluble fraction under high Cd concentrations. In leaves, a majority of Cd was accumulated in the cell wall regardless of Cd treatment level. Addition of SNP at 250 µM significantly alleviated Cd toxicity in peanut seedlings, including improved photosynthesis, up-regulated antioxidant system, and reduced Cd translocation from roots to shoots as evidenced by decreased Cd accumulation in stems and leaves. SNP application also changed the subcellular distribution of Cd in leaf and root tissues, by increasing Cd retention in root and leaf cell wall while reducing Cd accumulation in the soluble fractions and cell organelles. These results indicate that SNP has great application potential for improving the growth of plants under heavy metal stress such as Cd toxicity.

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Acknowledgments

The authors thank lecturer Xiujuan Wang (College of Foreign Languages, Shandong Agricultural University, Shandong, China) for her critical reading and revision of the manuscript. Special acknowledgements are given to the editors and reviewers. Great thanks were given to Pingping Yang, College of Animal Science Technology, Shandong Agricultural University, Shandong, China, for supplying instruments and patient guidance. This research work was financially supported by a Project of Shandong Province Higher Educational Science and Technology Program (J14LF08), the Chinese National Basic Research Program (2015CB150404) and the Shandong Provincial Natural Science Foundation of China (ZR2013CM003).

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Authors and Affiliations

  1. College of Resources and Environment, Shandong Agricultural University, Tai’an, Shandong, 271018, People’s Republic of China

    Yuanjie Dong, Weifeng Chen, Linlin Xu, Jing Kong & Shuang Liu

  2. Institute of Food and Agricultural Sciences, Indian River Research and Education Center, University of Florida, 2199 S. Rock Road, Fort Pierce, FL, 34945, USA

    Zhenli He

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  1. Yuanjie Dong
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  2. Weifeng Chen
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  3. Linlin Xu
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  4. Jing Kong
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  5. Shuang Liu
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Correspondence to Weifeng Chen.

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Dong, Y., Chen, W., Xu, L. et al. Nitric oxide can induce tolerance to oxidative stress of peanut seedlings under cadmium toxicity. Plant Growth Regul 79, 19–28 (2016). https://doi.org/10.1007/s10725-015-0105-3

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  • DOI: https://doi.org/10.1007/s10725-015-0105-3

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