Abstract
Parthenocarpy largely depends on the coordinated action of hormones produced in unpollinated ovaries, but can be induced by application of exogenous hormones. We evaluated the effects of gibberellins (GA4+7) and 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU) on induction and quality of parthenocarpic fruit in Pyrus pyrifolia Nakai ‘Cuiguan’ pear. Parthenocarpic fruit with a small core and a high edible ratio were induced by GA4+7 and/or CPPU. GA4+7 application induced normally shaped fruit with superior quality and normal size, whereas CPPU treatments resulted in abnormally shaped fruit with a larger size and an extraordinarily expanded calyx tube. Among all GA4+7 treatments, 500 mg L−1 GA4+7 induced the highest fruit set (91.88 %) and increased fruit size by 85 % compared with fruit induced by 200 mg L−1 GA4+7. In addition, the parthenocarpic fruit induced by GA4+7 accumulated considerably higher quantities of sucrose and less organic acids than pollinated and CPPU-induced fruit. The potential commercial application of CPPU and GA to pear in place of hand pollination is discussed.
Similar content being viewed by others
References
Aslmoshtaghi E, Shahsavar A (2013) Study on the induction of seedless loquat. Thai J Agric Sci 46:53–57
Bangerth F, Schröder M (1994) Strong synergistic effects of gibberellins with the synthetic cytokinin N-(2-chloro-4-pyridyl)-N-phenylurea on parthenocarpic fruit set and some other fruit characteristics of apple. Plant Growth Reg 15:293–302
Bologa KL, Fernie AR, Leisse A, Loureiro ME, Geigenberger P (2003) A bypass of sucrose synthase leads to low internal oxygen and impaired metabolic performance in growing potato tubers. Plant Physiol 132:2058–2072
Bukovac MJ (1963) Induction of parthenocarpic growth of apple fruits with gibberellins A3 and A4. Bot Gaz 124:191–195
Carmi N, Salts Y, Dedicova B, Shabtai S, Barg R (2003) Induction of parthenocarpy in tomato via specific expression of the rolB gene in the ovary. Planta 217:726–735
Costa F, Alba R, Schouten H, Soglio V, Gianfranceschi L, Serra S, Musacchi S, Sansavini S, Costa G, Fei Z (2010) Use of homologous and heterologous gene expression profiling tools to characterize transcription dynamics during apple fruit maturation and ripening. BMC Plant Biol 10:229
Crane JC, Primer PE, Campbell RC (1960) Gibberellin induced parthenocarpy in Prunus. Proc Am Soc Hortic Sci 75:129–137
Davison RM (1960) Fruit-setting of apples using gibberellic acid. Nature 188:681–682
Ding J, Chen B, Xia X, Mao W, Shi K, Zhou Y, Yu J (2013) Cytokinin-induced parthenocarpic fruit development in tomato is partly dependent on enhanced gibberellin and auxin biosynthesis. PLoS One 8(7):e70080
Eriksson S, Bohlenius H, Moritz T, Nilsson O (2006) GA4 is the active gibberellin in the regulation of LEAFY transcription and Arabidopsis floral initiation. Plant Cell 18:2172–2181
Gustafson FG (1942) Parthenocarpy: natural and artificial. Bot Rev 8:599–654
Hackel A, Schauer N, Carrari F, Fernie AR, Grimm B, Kühn C (2006) Sucrose transporter LeSUT1 and LeSUT2 inhibition affects tomato fruit development in different ways. Plant J 45:180–192
Huang C, Yu B, Teng Y, Su J, Shu Q, Cheng Z, Zeng L (2009) Effects of fruit bagging on coloring and related physiology, and qualities of red Chinese sand pears during fruit maturation. Sci Hortic 121:149–158
Iwahori S, Tominaga S, Yamasaki T (1988) Stimulation of fruit growth of kiwifruit, Actinidia chinensis Planch., by N-(2-chloro-4-pyridyl)-N’-phenylurea, a diphenylurea-derivative cytokinin. Sci Hortic 35:109–115
Khan MR, Hu J, He C (2012) Plant hormones including ethylene are recruited in calyx inflation in solanaceous plants. J Plant Physiol 169:940–948
Komatsu A, Takanokura Y, Moriguchi T, Omura M, Akihama T (1999) Differential expression of three sucrose-phosphate synthase isoforms during sucrose accumulation in citrus fruits (Citrus unshiu Marc.). Plant Sci 140:169–178
Pegoraro C, Chaves FC, Dal Cero J, Girardi CL, Rombaldi CV (2011) Effects of pre-harvest gibberellic acid spraying on gene transcript accumulation during peach fruit development. Plant Growth Reg 65:231–237
Ruan YL, Jin Y, Yang YJ, Li GJ, Boyer JS (2010) Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Mol Plant 3:942–955
Ruan YL, Patrick JW, Bouzayen M, Osorio S, Fernie AR (2012) Molecular regulation of seed and fruit set. Trends Plant Sci 17:656–665
Schwabe WW, Mills JJ (1981) Hormones and parthenocarpic fruit set: a literature survey. Hortic Abstr 51:661–699
Seehuber C, Damerow L, Blanke M (2011) Regulation of source: sink relationship, fruit set, fruit growth and fruit quality in European plum (Prunus domestica L.) using thinning for crop load management. Plant Growth Reg 652:335–341
Serrani JC, Sanjuan R, Ruiz-Rivero O, FosSerrani JC, Sanjuán R, Ruiz-Rivero O, Fos M, García-Martínez JL (2007) Gibberellin regulation of fruit set and growth in tomato. Plant Physiol 145:246–257
Shiomi S, Kubo Y, Wamocho LS, Koaze H, Nakamura R, Inaba A (1996) Postharvest ripening and ethylene biosynthesis in purple passion fruit. Postharvest Biol Technol 8:199–207
Shishido Y, Hori Y, Shikano S (1990) Effects of benzyladenine on translocation and distribution of photoassimilates during fruit setting and development in cucumber plants. J Jpn Soc Hortic Sci 59:129–136
Silva JM, Barba NG, Barros MT, Torres-Paulo A (2005) ‘Rocha’, the pear from Portugal. Acta Hortic 671:219–222
Tsaniklidis G, Delis C, Liakopoulos G, Karapanos I, Katinakis P, Passam HC, Aivalakis G (2012) Induced parthenocarpic cherry tomato fruits did not shown significant differences in l-ascorbate content but showed different pattern in GalLDH and GME expression. Plant Growth Reg 68:493–502
Tsaniklidis G, Delis C, Nikoloudakis N, Katinakis P, Passam HC, Aivalakis G (2014) l-Ascorbic acid metabolism in parthenocarpic and seeded cherry tomatoes. Plant Growth Reg 72:141–153
Wang H, Schauer N, Usadel B, Frasse P, Zouine M, Hernould M, Latche A, Pech JC, Fernie AR, Bouzayen M (2005) The tomato Aux/IAA transcription factor IAA9 is involved in fruit development and leaf morphogenesis. Plant Cell 17:2676–2692
Wang H, Schauer N, Usadel B, Frasse P, Zouine M, Hernould M, Latche A, Pech JC, Fernie AR, Bouzayen M (2009) Regulatory features underlying pollination-dependent and -independent tomato fruit set revealed by transcript and primary metabolite profiling. Plant Cell 21:1428–1452
Watanabe M, Segawa H, Murakami M, Sagawa S, Sadao K (2008) Effects of plant growth regulators on fruit set and fruit shape of parthenocarpic apple fruits. J Jpn Soc Hortic Sci 77:350–357
Yarushnykov VV, Blanke MM (2005) Alleviation of frost damage to pear flowers by application of gibberellin. Plant Growth Reg 45:21–27
Zhang C, Lee U, Tanabe K (2008) Hormonal regulation of fruit set, parthenogenesis induction and fruit expansion in Japanese pear. Plant Growth Reg 55:231–240
Zhang D, Qian M, Yu B, Teng Y (2013) Effect of fruit maturity on UV-B-induced post-harvest anthocyanin accumulation in red Chinese sand pear. Acta Physiol Plant 35:2857–2866
Zhu Y, Zheng P, Varanasi V, Shin S, Main D, Curry E, Mattheis JP (2012) Multiple plant hormones and cell wall metabolism regulate apple fruit maturation patterns and texture attributes. Tree Genet Genomes 8:1389–1406
Acknowledgments
This work was financed by the Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-29).
Author information
Authors and Affiliations
Corresponding author
Electronic supplementary material
Below is the link to the electronic supplementary material.
Rights and permissions
About this article
Cite this article
Niu, Q., Wang, T., Li, J. et al. Effects of exogenous application of GA4+7 and N-(2-chloro-4-pyridyl)-N′-phenylurea on induced parthenocarpy and fruit quality in Pyrus pyrifolia ‘Cuiguan’. Plant Growth Regul 76, 251–258 (2015). https://doi.org/10.1007/s10725-014-9995-8
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10725-014-9995-8