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Changes in H+-ATPase activity and conjugated polyamine contents in plasma membrane purified from developing wheat embryos under short-time drought stress

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Abstract

Plasma membrane (PM) vesicles were isolated from developing embryos of wheat (Triticum aestivum L.) with different drought-tolerance under drought stress by the gradient centrifugation method. The activity of the PM H+-ATPase (EC 3.6.1.35) and contents of polyamine conjugated (covalently and noncovalently) to the PM vesicles were investigated. Results showed that after drought treatment for 3 d, embryo relative water content (ERWC), embryo relative dry weight increase rate (ERDWIR) of drought-sensitive Yumai No. 48 cultivar decreased more significantly than those of drought-tolerant Luomai No. 22 cultivar, while PM H+-ATPase activity, noncovalently conjugated (NCC) spermidine (Spd) and NCC spermine (Spm), the covalently conjugated (CC) putrescine (Put) and CC Spd of PM from Luomai No. 22 cultivar increased more obviously than those from Yumai No. 48 cultivar. As judged by increases in ERWC and ERDWIR, treatment with exogenous Spd alleviated markedly drought injuries to Yumai No. 48, coupled with significant increases in NCC Spd and NCC Spm levels and H+-ATPase activity in the embryo PM vesicle. Under drought stress, the treatment of drought-tolerant Luomai No. 22 cultivar with methylglyoxyl-bis (guanylhydrazone) (MGBG), an inhibitor of S-adenosylmethionine decarboxylase (SAMDC), and phenanthrolin (o−Phen), an inhibitor of transglutaminase (TGase) respectively, caused a decrease of the NCC Spd, NCC Spm, CC Put and CC Spd. Those decreases were associated with decreased PM-H+-ATPase activity and the tolerance of developing wheat embryos to osmotic stress, as judged by decreases in ERWC and ERDWIR. These results suggest that tolerance of the developing wheat embryos to drought stress is associated with the embryo PM H+-ATPase and the levels of NCC Spd, NCC Spm, CC Put and CC Spd in embryo PM vesicles.

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Abbreviations

BSA:

Bovine serum albumin

CC:

Covalently conjugated

DR:

Drought

DTT:

Dithiothreitol

EGTA:

Ethylene glycol tetraacetic acid

ERDWIR:

Embryo relative dry weight increase rate

ERWC:

Embryo relative water content

Hepes:

2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid

HPLC:

High performance liquid chromatography

MGBG:

Methylglyoxyl-bis (guanylhydrazone)

NCC:

Noncovalently conjugated

O−Phen:

Phenanthrolin

PA:

Polyamine

PCA:

Perchloric acid

PEG:

Polyethylene glycol–6000

PM:

Plasma membrane

PMSF:

Phenylmethanesulfonyl fluoride

Put:

Putrescine

PVP:

Polyvinyl pyrrolidone

SAMDC:

S-adenosylmethionine decarboxylase

Spd:

Spermidine

Spm:

Spermine

TCA:

Trichloroacetic acid

TGase:

Transglutaminase

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Acknowledgments

This research was supported by the National Natural Science Foundation of China (Grant No. 31271627), the Special Fund of Industrial (Agriculture) Research for Public Welfare of China (Grant No. 201203077) and the Education Department Natural Science Foundation of Henan Province (Grant No. 2011A180033).

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Authors and Affiliations

  1. The Collaborative Innovation Center of Henan Grain Crops, the National Key Laboratory of Wheat and Maize Crop Science, the College of Agronomy, Henan Agricultural University, Zhengzhou, 450002, Henan, China

    Hongyang Du & Qinghua Yang

  2. College of Life Science and Agronomy, Zhoukou Normal University, Zhoukou, 466001, Henan, China

    Hongyang Du, Huaipan Liu & Ronald Kurtenbach

  3. Farmland Irrigation Research Institute, Chinese Academy of Agriculture Science, Xinxiang, 453002, Henan, China

    Xinguo Zhou

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  1. Hongyang Du
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  2. Xinguo Zhou
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  3. Qinghua Yang
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Correspondence to Qinghua Yang.

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Du, H., Zhou, X., Yang, Q. et al. Changes in H+-ATPase activity and conjugated polyamine contents in plasma membrane purified from developing wheat embryos under short-time drought stress. Plant Growth Regul 75, 1–10 (2015). https://doi.org/10.1007/s10725-014-9925-9

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