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Symbiotic effectiveness and plant growh promoting traits in some Rhizobium strains isolated from Phaseolus vulgaris L.

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Abstract

The ability of Rhizobia to colonize roots of certain legumes and promote their growth has been proven previously. In this study the symbiotic efficiency of 47 Rhizobium strains with 6 common bean cultivars was evaluated under greenhouse condition. Fourteen strains showed the best symbiotic efficiency, whereas some isolates could not induce nodules on host plants. The ability of fourteen superior strains to solubilize phosphorus and zinc and to produce auxin, HCN and siderohores was evaluated in the laboratory assays. Rhizobium strain Rb102 produced the highest amount of auxin (14.2 mg l−1) in the medium containing l-tryptophan. None of the isolates were able to solubilize ZnO and ZnCO3 on solid medium but in liquid medium some of them had negligible solubilization. The highest P solubility in liquid and solid medium was observed in strains Rb113 and Rb130, respectively. Strain Rb102 produced the highest amount of siderophores. None of the isolates were able to produce HCN. This study showed that there was a great diversity between the strains of Rhizobium in terms of their plant growth promoting traits symbiotic efficiency which supports the importance of screening rhizobia for selecting the most efficient strains. The genetic diversity of the isolates was analyzed by PCR–RFLP of the 16S rDNA. Our rhizobia were clustered into 10 groups showing high levels of diversity.

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Authors and Affiliations

  1. Department of Soil Science, Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    P. Abbaszadeh-dahaji, Gh. R. Savaghebi, M. Farahbakhsh & B. Moteshareh-zadeh

  2. Department of Soil Biology, Soil and Water Research Institute, Karaj, Iran

    H. Asadi-rahmani & F. Rejali

  3. Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran

    M. Omidvari

  4. Department of Applied Chemistry and Microbiology, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, Viikki Biocenter, University of Helsinki, Helsinki, Finland

    K. Lindstrom

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  1. P. Abbaszadeh-dahaji
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  2. Gh. R. Savaghebi
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  3. H. Asadi-rahmani
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Correspondence to P. Abbaszadeh-dahaji.

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Abbaszadeh-dahaji, P., Savaghebi, G.R., Asadi-rahmani, H. et al. Symbiotic effectiveness and plant growh promoting traits in some Rhizobium strains isolated from Phaseolus vulgaris L.. Plant Growth Regul 68, 361–370 (2012). https://doi.org/10.1007/s10725-012-9724-0

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  • DOI: https://doi.org/10.1007/s10725-012-9724-0

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